2tbs

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[[Image:2tbs.jpg|left|200px]]<br /><applet load="2tbs" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2tbs.jpg|left|200px]]
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caption="2tbs, resolution 1.8&Aring;" />
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'''COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS'''<br />
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{{Structure
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|PDB= 2tbs |SIZE=350|CAPTION= <scene name='initialview01'>2tbs</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]
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|GENE=
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}}
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'''COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2TBS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=BEN:'>BEN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1TBS. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBS OCA].
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2TBS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. This structure supersedes the now removed PDB entry 1TBS. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBS OCA].
==Reference==
==Reference==
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Cold adaption of enzymes: structural comparison between salmon and bovine trypsins., Smalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R, Proteins. 1994 Oct;20(2):149-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7846025 7846025]
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Cold adaption of enzymes: structural comparison between salmon and bovine trypsins., Smalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R, Proteins. 1994 Oct;20(2):149-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7846025 7846025]
[[Category: Salmo salar]]
[[Category: Salmo salar]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: hydrolase(serine proteinase)]]
[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:38:53 2008''

Revision as of 16:38, 20 March 2008


PDB ID 2tbs

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands: and
Activity: Trypsin, with EC number 3.4.21.4
Coordinates: save as pdb, mmCIF, xml



COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS


Overview

The crystal structure of an anionic form of salmon trypsin has been determined at 1.82 A resolution. We report the first structure of a trypsin from a phoikilothermic organism in a detailed comparison to mammalian trypsins in order to look for structural rationalizations for the cold-adaption features of salmon trypsin. This form of salmon trypsin (ST II) comprises 222 residues, and is homologous to bovine trypsin (BT) in about 65% of the primary structure. The tertiary structures are similar, with an overall displacement in main chain atomic positions between salmon trypsin and various crystal structures of bovine trypsin of about 0.8 A. Intramolecular hydrogen bonds and hydrophobic interactions are compared and discussed in order to estimate possible differences in molecular flexibility which might explain the higher catalytic efficiency and lower thermostability of salmon trypsin compared to bovine trypsin. No overall differences in intramolecular interactions are detected between the two structures, but there are differences in certain regions of the structures which may explain some of the observed differences in physical properties. The distribution of charged residues is different in the two trypsins, and the impact this might have on substrate affinity has been discussed.

About this Structure

2TBS is a Single protein structure of sequence from Salmo salar. This structure supersedes the now removed PDB entry 1TBS. Full crystallographic information is available from OCA.

Reference

Cold adaption of enzymes: structural comparison between salmon and bovine trypsins., Smalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R, Proteins. 1994 Oct;20(2):149-66. PMID:7846025

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