2tsb
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2tsb.jpg|left|200px]] | + | [[Image:2tsb.jpg|left|200px]] |
| - | + | ||
| - | '''AZURIN MUTANT M121A-AZIDE''' | + | {{Structure |
| + | |PDB= 2tsb |SIZE=350|CAPTION= <scene name='initialview01'>2tsb</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene> and <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''AZURIN MUTANT M121A-AZIDE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2TSB is a [ | + | 2TSB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. This structure supersedes the now removed PDB entry 1TSB. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TSB OCA]. |
==Reference== | ==Reference== | ||
| - | Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties., Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:[http:// | + | Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties., Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299604 15299604] |
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:39:31 2008'' |
Revision as of 16:39, 20 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
AZURIN MUTANT M121A-AZIDE
Overview
The crystal structures of the azurin mutant Met121Ala and its azide derivative Met121Ala-azide from Pseudomonas aeruginosa have been determined. The final crystallographic R values are 21.3 and 19.4% for the two structures, respectively. In the Met121Ala mutant, the distance between the copper ion and His117 increases by 0.34 A compared with the wild-type structure. The removal of the methionine in the apical position induces a shortening of the distance from the copper ion to the carbonyl O atom of Gly45 from 2.97 to 2.74 A. In the Met121Ala-azide structure, the azide anion occupies the cavity created by replacing the Met121 side chain with the smaller methyl group of Ala. The azide anion binds with a terminal N atom to the copper ion at a distance of about 2.04 A. In addition, the copper ion has moved out of the trigonal plane by about 0.26 A towards the azide anion. Thus, the copper site in this structure has a distorted tetrahedral arrangement. The spectroscopic characteristics show, in addition, that the copper sites in the two structures are distinctively different. The Met121Ala mutant still maintains the properties of an ordinary type 1 copper site while the Met121Ala-azide derivative has an absorption maximum at about 409 nm and the copper hyperfine coupling has increased to a value intermediate between those of type 2 copper and the wild-type azurin.
About this Structure
2TSB is a Single protein structure of sequence from Pseudomonas aeruginosa. This structure supersedes the now removed PDB entry 1TSB. Full crystallographic information is available from OCA.
Reference
Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties., Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:15299604
Page seeded by OCA on Thu Mar 20 18:39:31 2008
