2uu9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2uu9.gif|left|200px]]<br /><applet load="2uu9" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2uu9.gif|left|200px]]
-
caption="2uu9, resolution 3.10&Aring;" />
+
 
-
'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT COMPLEXED WITH A VALINE-ASL WITH CMO5U IN POSITION 34 BOUND TO AN MRNA WITH A GUG-CODON IN THE A-SITE AND PAROMOMYCIN.'''<br />
+
{{Structure
 +
|PDB= 2uu9 |SIZE=350|CAPTION= <scene name='initialview01'>2uu9</scene>, resolution 3.10&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT COMPLEXED WITH A VALINE-ASL WITH CMO5U IN POSITION 34 BOUND TO AN MRNA WITH A GUG-CODON IN THE A-SITE AND PAROMOMYCIN.'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2UU9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UU9 OCA].
+
2UU9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UU9 OCA].
==Reference==
==Reference==
-
Mechanism for expanding the decoding capacity of transfer RNAs by modification of uridines., Weixlbaumer A, Murphy FV 4th, Dziergowska A, Malkiewicz A, Vendeix FA, Agris PF, Ramakrishnan V, Nat Struct Mol Biol. 2007 Jun;14(6):498-502. Epub 2007 May 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17496902 17496902]
+
Mechanism for expanding the decoding capacity of transfer RNAs by modification of uridines., Weixlbaumer A, Murphy FV 4th, Dziergowska A, Malkiewicz A, Vendeix FA, Agris PF, Ramakrishnan V, Nat Struct Mol Biol. 2007 Jun;14(6):498-502. Epub 2007 May 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17496902 17496902]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
Line 27: Line 36:
[[Category: coiled coil]]
[[Category: coiled coil]]
[[Category: metal-binding]]
[[Category: metal-binding]]
-
[[Category: modifiactions]]
+
[[Category: modifiaction]]
[[Category: mrna]]
[[Category: mrna]]
[[Category: paromomycin]]
[[Category: paromomycin]]
Line 41: Line 50:
[[Category: zinc-finger]]
[[Category: zinc-finger]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:50:17 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:39:47 2008''

Revision as of 16:39, 20 March 2008

Image:2uu9.gif


PDB ID 2uu9

Drag the structure with the mouse to rotate
, resolution 3.10Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT COMPLEXED WITH A VALINE-ASL WITH CMO5U IN POSITION 34 BOUND TO AN MRNA WITH A GUG-CODON IN THE A-SITE AND PAROMOMYCIN.


Overview

One of the most prevalent base modifications involved in decoding is uridine 5-oxyacetic acid at the wobble position of tRNA. It has been known for several decades that this modification enables a single tRNA to decode all four codons in a degenerate codon box. We have determined structures of an anticodon stem-loop of tRNA(Val) containing the modified uridine with all four valine codons in the decoding site of the 30S ribosomal subunit. An intramolecular hydrogen bond involving the modification helps to prestructure the anticodon loop. We found unusual base pairs with the three noncomplementary codon bases, including a G.U base pair in standard Watson-Crick geometry, which presumably involves an enol form for the uridine. These structures suggest how a modification in the uridine at the wobble position can expand the decoding capability of a tRNA.

About this Structure

2UU9 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Mechanism for expanding the decoding capacity of transfer RNAs by modification of uridines., Weixlbaumer A, Murphy FV 4th, Dziergowska A, Malkiewicz A, Vendeix FA, Agris PF, Ramakrishnan V, Nat Struct Mol Biol. 2007 Jun;14(6):498-502. Epub 2007 May 13. PMID:17496902

Page seeded by OCA on Thu Mar 20 18:39:47 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uu9"
Personal tools