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2uuu
From Proteopedia
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| - | [[Image:2uuu.gif|left|200px]] | + | [[Image:2uuu.gif|left|200px]] |
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| - | '''ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P212121''' | + | {{Structure |
| + | |PDB= 2uuu |SIZE=350|CAPTION= <scene name='initialview01'>2uuu</scene>, resolution 1.95Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Pl3+Binding+Site+For+Chain+D'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=PL3:HEXADECAN-1-OL'>PL3</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alkylglycerone-phosphate_synthase Alkylglycerone-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.26 2.5.1.26] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P212121''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2UUU is a [ | + | 2UUU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUU OCA]. |
==Reference== | ==Reference== | ||
| - | The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:[http:// | + | The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17562315 17562315] |
[[Category: Alkylglycerone-phosphate synthase]] | [[Category: Alkylglycerone-phosphate synthase]] | ||
[[Category: Dictyostelium discoideum]] | [[Category: Dictyostelium discoideum]] | ||
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[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: PL3]] | [[Category: PL3]] | ||
| - | [[Category: biosynthesis of | + | [[Category: biosynthesis of phospholipid]] |
[[Category: fad]] | [[Category: fad]] | ||
[[Category: flavin]] | [[Category: flavin]] | ||
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[[Category: peroxisomal disorder]] | [[Category: peroxisomal disorder]] | ||
[[Category: peroxisome]] | [[Category: peroxisome]] | ||
| - | [[Category: | + | [[Category: plasmalogen]] |
[[Category: rhizomelic chondrodysplasia punctata]] | [[Category: rhizomelic chondrodysplasia punctata]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:39:57 2008'' |
Revision as of 16:39, 20 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Alkylglycerone-phosphate synthase, with EC number 2.5.1.26 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE IN P212121
Overview
Ether phospholipids are essential constituents of eukaryotic cell membranes. Rhizomelic chondrodysplasia punctata type 3 is a severe peroxisomal disorder caused by inborn deficiency of alkyldihydroxyacetonephosphate synthase (ADPS). The enzyme carries out the most characteristic step in ether phospholipid biosynthesis: formation of the ether bond. The crystal structure of ADPS from Dictyostelium discoideum shows a fatty-alcohol molecule bound in a narrow hydrophobic tunnel, specific for aliphatic chains of 16 carbons. Access to the tunnel is controlled by a flexible loop and a gating helix at the protein-membrane interface. Structural and mutagenesis investigations identify a cluster of hydrophilic catalytic residues, including an essential tyrosine, possibly involved in substrate proton abstraction, and the arginine that is mutated in ADPS-deficient patients. We propose that ether bond formation might be orchestrated through a covalent imine intermediate with the flavin, accounting for the noncanonical employment of a flavin cofactor in a nonredox reaction.
About this Structure
2UUU is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
Reference
The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder., Razeto A, Mattiroli F, Carpanelli E, Aliverti A, Pandini V, Coda A, Mattevi A, Structure. 2007 Jun;15(6):683-92. PMID:17562315
Page seeded by OCA on Thu Mar 20 18:39:57 2008
Categories: Alkylglycerone-phosphate synthase | Dictyostelium discoideum | Single protein | Aliverti, A. | Carpanelli, E. | Coda, A. | Mattevi, A. | Mattiroli, F. | Pandini, V. | Razeto, A. | FAD | PL3 | Biosynthesis of phospholipid | Fad | Flavin | Flavoprotein | Lipid synthesis | Peroxisomal disorder | Peroxisome | Plasmalogen | Rhizomelic chondrodysplasia punctata | Transferase
