2v0o
From Proteopedia
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| - | [[Image:2v0o.gif|left|200px]] | + | [[Image:2v0o.gif|left|200px]] |
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| - | '''FCHO2 F-BAR DOMAIN''' | + | {{Structure |
| + | |PDB= 2v0o |SIZE=350|CAPTION= <scene name='initialview01'>2v0o</scene>, resolution 2.30Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+C'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''FCHO2 F-BAR DOMAIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V0O is a [ | + | 2V0O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0O OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and analysis of FCHo2 F-BAR domain: a dimerizing and membrane recruitment module that effects membrane curvature., Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT, Structure. 2007 Jul;15(7):839-52. Epub 2007 Jun 1. PMID:[http:// | + | Structure and analysis of FCHo2 F-BAR domain: a dimerizing and membrane recruitment module that effects membrane curvature., Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT, Structure. 2007 Jul;15(7):839-52. Epub 2007 Jun 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17540576 17540576] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vesicle trafficking]] | [[Category: vesicle trafficking]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:42:00 2008'' |
Revision as of 16:42, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
FCHO2 F-BAR DOMAIN
Overview
A spectrum of membrane curvatures exists within cells, and proteins have evolved different modules to detect, create, and maintain these curvatures. Here we present the crystal structure of one such module found within human FCHo2. This F-BAR (extended FCH) module consists of two F-BAR domains, forming an intrinsically curved all-helical antiparallel dimer with a Kd of 2.5 microM. The module binds liposomes via a concave face, deforming them into tubules with variable diameters of up to 130 nm. Pulse EPR studies showed the membrane-bound dimer is the same as the crystal dimer, although the N-terminal helix changed conformation on membrane binding. Mutation of a phenylalanine on this helix partially attenuated narrow tubule formation, and resulted in a gain of curvature sensitivity. This structure shows a distant relationship to curvature-sensing BAR modules, and suggests how similar coiled-coil architectures in the BAR superfamily have evolved to expand the repertoire of membrane-sculpting possibilities.
About this Structure
2V0O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and analysis of FCHo2 F-BAR domain: a dimerizing and membrane recruitment module that effects membrane curvature., Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT, Structure. 2007 Jul;15(7):839-52. Epub 2007 Jun 1. PMID:17540576
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