2mxd

Structural highlights

Function

[POLG_PESV] NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity (By similarity). Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation (By similarity). Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved (By similarity). It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs (By similarity). Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm (By similarity).

References

1. ↑ Hwang HJ, Min HJ, Yun H, Pelton JG, Wemmer DE, Cho KO, Kim JS, Lee CW. Solution structure of the porcine sapovirus VPg core reveals a stable three-helical bundle with a conserved surface patch. Biochem Biophys Res Commun. 2015 Apr 17;459(4):610-6. doi:, 10.1016/j.bbrc.2015.02.156. Epub 2015 Mar 6. PMID:25753201 doi:http://dx.doi.org/10.1016/j.bbrc.2015.02.156