4plj
From Proteopedia
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| - | ''' | + | ==Hepatitis E Virus E2s domain (Genotype IV) in complex with a neutralizing antibody 8G12== |
| + | <StructureSection load='4plj' size='340' side='right' caption='[[4plj]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4plj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4plk|4plk]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4plj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4plj RCSB], [http://www.ebi.ac.uk/pdbsum/4plj PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hepatitis E virus (HEV), a non-enveloped, positive-sense, single-stranded RNA virus, is a major cause of enteric hepatitis. Classified into the family Hepeviridae, HEV comprises four genotypes (genotypes 1-4), which belong to a single serotype. We describe a monoclonal antibody (mAb), 8G12, which equally recognizes all four genotypes of HEV, with approximately 2.53-3.45 nM binding affinity. The mAb 8G12 has a protective, neutralizing capacity, which can significantly block virus infection in host cells. Animal studies with genotypes 1, 3 and 4 confirmed the cross-genotype neutralizing capacity of 8G12 and its effective prevention of hepatitis E disease. The complex crystal structures of 8G12 with the HEV E2s domain (the most protruded region of the virus capsid) of the abundant genotypes 1 and 4 were determined at 4.0 and 2.3 A resolution, respectively. These structures revealed that 8G12 recognizes both genotypes through the epitopes in the E2s dimerization region. Structure-based mutagenesis and cell-model assays with virus-like particles identified several conserved residues (Glu549, Lys554 and Gly591) that are essential for 8G12 neutralization. Moreover, the epitope of 8G12 is identified as a key epitope involved in virus-host interactions. These findings will help develop a common strategy for the prevention of the most abundant form of HEV infection.Cell Research advance online publication 20 March 2015; doi:10.1038/cr.2015.34. | ||
| - | + | Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody.,Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S, Xia N, Sivaraman J Cell Res. 2015 Mar 20. doi: 10.1038/cr.2015.34. PMID:25793314<ref>PMID:25793314</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Li, S W]] | ||
[[Category: Sivaraman, J]] | [[Category: Sivaraman, J]] | ||
| - | [[Category: | + | [[Category: Tang, X H]] |
| + | [[Category: Complex]] | ||
| + | [[Category: Neutralizing antibody]] | ||
| + | [[Category: Viral protein-immume system complex]] | ||
Revision as of 12:48, 15 April 2015
Hepatitis E Virus E2s domain (Genotype IV) in complex with a neutralizing antibody 8G12
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