2v2k

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[[Image:2v2k.jpg|left|200px]]<br /><applet load="2v2k" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2v2k.jpg|left|200px]]
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caption="2v2k, resolution 1.60&Aring;" />
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'''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''<br />
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{{Structure
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|PDB= 2v2k |SIZE=350|CAPTION= <scene name='initialview01'>2v2k</scene>, resolution 1.60&Aring;
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|SITE= <scene name='pdbsite=AC1:F3s+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=F3S:FE3-S4 CLUSTER'>F3S</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:F3s+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA].
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2V2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA].
==Reference==
==Reference==
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The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17577575 17577575]
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The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17577575 17577575]
[[Category: Mycobacterium smegmatis]]
[[Category: Mycobacterium smegmatis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: ACT]]
[[Category: ACT]]
[[Category: F3S]]
[[Category: F3S]]
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[[Category: 3fe-4s]]
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[[Category: 3fe-4]]
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[[Category: 4fe-4s]]
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[[Category: 4fe-4]]
[[Category: 4fe-4s cluster instability]]
[[Category: 4fe-4s cluster instability]]
[[Category: 7fe ferredoxin]]
[[Category: 7fe ferredoxin]]
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[[Category: iron-sulfur]]
[[Category: iron-sulfur]]
[[Category: metal-binding]]
[[Category: metal-binding]]
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[[Category: mycobacterium smegmatis]]
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[[Category: mycobacterium smegmati]]
[[Category: mycobacterium tuberculosis]]
[[Category: mycobacterium tuberculosis]]
[[Category: transport]]
[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:52:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:42:39 2008''

Revision as of 16:42, 20 March 2008

Image:2v2k.jpg


PDB ID 2v2k

Drag the structure with the mouse to rotate
, resolution 1.60Å
Sites:
Ligands: and
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS


Overview

Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S] and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A. Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting that M. tuberculosis FdxC could be the physiological partner of the enzyme in providing reducing power to the cytochromes P450. We report here the crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free) 20.2%). Besides providing an insight on protein architecture for this 106-residue ferredoxin, our crystallographic investigation highlights lability of the [4Fe-4S] center, which is shown to loose a Fe atom during crystal growth. Due to their high similarity (87% sequence identity), the structure here reported can be considered a valuable model for M. tuberculosis FdxC, thus representing a step forward in the study of the complex mycobacterial redox pathways.

About this Structure

2V2K is a Single protein structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.

Reference

The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis., Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M, Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575

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