2v9f

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[[Image:2v9f.jpg|left|200px]]<br /><applet load="2v9f" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2v9f.jpg|left|200px]]
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caption="2v9f, resolution 2.10&Aring;" />
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'''L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A-K248W-A273S)'''<br />
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{{Structure
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|PDB= 2v9f |SIZE=350|CAPTION= <scene name='initialview01'>2v9f</scene>, resolution 2.10&Aring;
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|SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Act+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Act+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Act+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Chain+A'>AC6</scene> and <scene name='pdbsite=AC7:Zn+Binding+Site+For+Chain+A'>AC7</scene>
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Rhamnulose-1-phosphate_aldolase Rhamnulose-1-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.19 4.1.2.19]
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|GENE=
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}}
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'''L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A-K248W-A273S)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2V9F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Rhamnulose-1-phosphate_aldolase Rhamnulose-1-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.19 4.1.2.19] Known structural/functional Sites: <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Act+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Act+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Act+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Chain+A'>AC6</scene> and <scene name='pdbsite=AC7:Zn+Binding+Site+For+Chain+A'>AC7</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9F OCA].
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2V9F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9F OCA].
==Reference==
==Reference==
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Designed protein-protein association., Grueninger D, Treiber N, Ziegler MO, Koetter JW, Schulze MS, Schulz GE, Science. 2008 Jan 11;319(5860):206-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18187656 18187656]
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Designed protein-protein association., Grueninger D, Treiber N, Ziegler MO, Koetter JW, Schulze MS, Schulz GE, Science. 2008 Jan 11;319(5860):206-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18187656 18187656]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Rhamnulose-1-phosphate aldolase]]
[[Category: Rhamnulose-1-phosphate aldolase]]
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[[Category: zinc enzyme]]
[[Category: zinc enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:44:32 2008''

Revision as of 16:44, 20 March 2008

Image:2v9f.jpg


PDB ID 2v9f

Drag the structure with the mouse to rotate
, resolution 2.10Å
Sites: , , , , , and
Ligands: and
Activity: Rhamnulose-1-phosphate aldolase, with EC number 4.1.2.19
Coordinates: save as pdb, mmCIF, xml



L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A-K248W-A273S)


Overview

The analysis of natural contact interfaces between protein subunits and between proteins has disclosed some general rules governing their association. We have applied these rules to produce a number of novel assemblies, demonstrating that a given protein can be engineered to form contacts at various points of its surface. Symmetry plays an important role because it defines the multiplicity of a designed contact and therefore the number of required mutations. Some of the proteins needed only a single side-chain alteration in order to associate to a higher-order complex. The mobility of the buried side chains has to be taken into account. Four assemblies have been structurally elucidated. Comparisons between the designed contacts and the results will provide useful guidelines for the development of future architectures.

About this Structure

2V9F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Designed protein-protein association., Grueninger D, Treiber N, Ziegler MO, Koetter JW, Schulze MS, Schulz GE, Science. 2008 Jan 11;319(5860):206-9. PMID:18187656

Page seeded by OCA on Thu Mar 20 18:44:32 2008

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