2v9y
From Proteopedia
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| - | [[Image:2v9y.jpg|left|200px]] | + | [[Image:2v9y.jpg|left|200px]] |
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| - | '''HUMAN AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE''' | + | {{Structure |
| + | |PDB= 2v9y |SIZE=350|CAPTION= <scene name='initialview01'>2v9y</scene>, resolution 2.10Å | ||
| + | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+B'>AC2</scene> and <scene name='pdbsite=AC3:So4+Binding+Site+For+Chain+B'>AC3</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoribosylformylglycinamidine_cyclo-ligase Phosphoribosylformylglycinamidine cyclo-ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.1 6.3.3.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HUMAN AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V9Y is a [ | + | 2V9Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9Y OCA]. |
==Reference== | ==Reference== | ||
| - | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http:// | + | In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17982461 17982461] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Phosphoribosylformylglycinamidine cyclo-ligase]] | [[Category: Phosphoribosylformylglycinamidine cyclo-ligase]] | ||
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[[Category: Welin, M.]] | [[Category: Welin, M.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| - | [[Category: | + | [[Category: air]] |
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
[[Category: aminoimidazole ribonucleotide synthetase]] | [[Category: aminoimidazole ribonucleotide synthetase]] | ||
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[[Category: purine metabolism]] | [[Category: purine metabolism]] | ||
[[Category: sgc]] | [[Category: sgc]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: structural genomics consortium]] | [[Category: structural genomics consortium]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:44:43 2008'' |
Revision as of 16:44, 20 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | |||||||
| Activity: | Phosphoribosylformylglycinamidine cyclo-ligase, with EC number 6.3.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2V9Y is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Thu Mar 20 18:44:43 2008
Categories: Homo sapiens | Phosphoribosylformylglycinamidine cyclo-ligase | Single protein | Arrowsmith, C H. | Berg, S Van Den. | Berglund, H. | Busam, R. | Collins, R. | Consortium, Structural Genomics. | Dahlgren, L G. | Edwards, A. | Flodin, S. | Flores, A. | Graslund, S. | Hallberg, B M. | Hammarstrom, M. | Herman, M D. | Holmberg-Schiavone, L. | Johansson, I. | Kallas, A. | Karlberg, T. | Kotenyova, T. | Lehtio, L. | Moche, M. | Nordlund, P. | Nyman, T. | Persson, C. | Sagemark, J. | Stenmark, P. | Sundstrom, M. | Thorsell, A G. | Tresaugues, L. | Weigelt, J. | Welin, M. | SO4 | Air | Alternative splicing | Aminoimidazole ribonucleotide synthetase | Atp-binding | Gart | Ligase | Multifunctional enzyme | Nucleotide-binding | Phosphorylation | Polymorphism | Purine biosynthesis | Purine metabolism | Sgc | Structural genomic | Structural genomics consortium | Transferase
