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4v3p
From Proteopedia
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| - | ''' | + | ==The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes== |
| + | <StructureSection load='4v3p' size='340' side='right' caption='[[4v3p]], [[Resolution|resolution]] 34.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4v3p]] is a 87 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V3P FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v3p RCSB], [http://www.ebi.ac.uk/pdbsum/4v3p PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During protein synthesis, several ribosomes bind to a single messenger RNA (mRNA) forming large macromolecular assemblies called polyribosomes. Here we report the detailed molecular structure of a 100 MDa eukaryotic poly-ribosome complex derived from cryo electron tomography, sub-tomogram averaging and pseudo-atomic modelling by crystal structure fitting. The structure allowed the visualization of the three functional parts of the polysome assembly, the central core region that forms a rather compact left-handed supra-molecular helix, and the more open regions that harbour the initiation and termination sites at either ends. The helical region forms a continuous mRNA channel where the mRNA strand bridges neighbouring exit and entry sites of the ribosomes and prevents mRNA looping between ribosomes. This structure provides unprecedented insights into protein- and RNA-mediated inter-ribosome contacts that involve conserved sites through 40S subunits and long protruding RNA expansion segments, suggesting a role in stabilizing the overall polyribosomal assembly. | ||
| - | The | + | The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes.,Myasnikov AG, Afonina ZA, Menetret JF, Shirokov VA, Spirin AS, Klaholz BP Nat Commun. 2014 Nov 7;5:5294. doi: 10.1038/ncomms6294. PMID:25376914<ref>PMID:25376914</ref> |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Triticum aestivum]] |
| - | [[Category: | + | [[Category: Afonina, z a]] |
| - | [[Category: | + | [[Category: Klaholz, b p]] |
| - | [[Category: | + | [[Category: Menetret, j f]] |
| + | [[Category: Myasnikov, A g]] | ||
| + | [[Category: Shirokov, v a]] | ||
| + | [[Category: Spirin, A s]] | ||
| + | [[Category: Ribosome]] | ||
Revision as of 12:38, 22 April 2015
The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes
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