4yna
From Proteopedia
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| - | ''' | + | ==Oxidized YfiR== |
| + | <StructureSection load='4yna' size='340' side='right' caption='[[4yna]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4yna]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yn7|4yn7]], [[4yn9|4yn9]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yna OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4yna RCSB], [http://www.ebi.ac.uk/pdbsum/4yna PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | YfiBNR is a recently identified c-di-GMP regulatory system involved in bacterial biofilm formation. The periplasmic protein YfiR inhibits the diguanylate cyclase activity of the inner membrane protein YfiN, whereas YfiB in the outer membrane can release this inhibition by sequestration of YfiR. In addition, this system may respond to anoxic conditions via YfiR, although the detailed mechanism is still unknown. Here we report crystal structures of Pseudomonas aeruginosa YfiR in the absence and presence of oxidative glutathione. Our structures reveal the overall folding of YfiR for the first time and demonstrate that YfiR exist as a dimer. Comparison of the two structures in different redox states revealed a broken/formation of one disulfide bond (Cys71-Cys110) and local conformational change around the other one (Cys145-Cys152). Mutagenesis studies indicated that Cys145-Cys152 plays an important role in maintaining the correct folding of YfiR. | ||
| - | + | Crystal structures of YfiR from Pseudomonas aeruginosa in two redox states.,Yang X, Yang XA, Xu M, Zhou L, Fan Z, Jiang T Biochem Biophys Res Commun. 2015 Apr 4. pii: S0006-291X(15)00628-2. doi:, 10.1016/j.bbrc.2015.03.160. PMID:25849887<ref>PMID:25849887</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Jiang, T]] | ||
[[Category: Xu, M]] | [[Category: Xu, M]] | ||
| - | [[Category: | + | [[Category: Oxidized form]] |
| + | [[Category: Periplasmic repressor protein]] | ||
Revision as of 12:46, 22 April 2015
Oxidized YfiR
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