2vfj

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[[Image:2vfj.jpg|left|200px]]<br /><applet load="2vfj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2vfj.jpg|left|200px]]
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caption="2vfj, resolution 3.2&Aring;" />
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'''STRUCTURE OF THE A20 OVARIAN TUMOUR (OTU) DOMAIN'''<br />
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{{Structure
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|PDB= 2vfj |SIZE=350|CAPTION= <scene name='initialview01'>2vfj</scene>, resolution 3.2&Aring;
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+C'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+D'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+D'>AC4</scene> and <scene name='pdbsite=AC5:Mg+Binding+Site+For+Chain+D'>AC5</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12]
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|GENE=
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}}
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'''STRUCTURE OF THE A20 OVARIAN TUMOUR (OTU) DOMAIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2VFJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12] Known structural/functional Sites: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+C'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+D'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+D'>AC4</scene> and <scene name='pdbsite=AC5:Mg+Binding+Site+For+Chain+D'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFJ OCA].
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2VFJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFJ OCA].
==Reference==
==Reference==
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Structure of the A20 OTU domain and mechanistic insights into deubiquitination., Komander D, Barford D, Biochem J. 2008 Jan 1;409(1):77-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17961127 17961127]
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Structure of the A20 OTU domain and mechanistic insights into deubiquitination., Komander D, Barford D, Biochem J. 2008 Jan 1;409(1):77-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17961127 17961127]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: zinc-finger]]
[[Category: zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:55:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:46:00 2008''

Revision as of 16:46, 20 March 2008

Image:2vfj.jpg


PDB ID 2vfj

Drag the structure with the mouse to rotate
, resolution 3.2Å
Sites: , , , and
Ligands: and
Activity: Ubiquitinyl hydrolase 1, with EC number 3.4.19.12
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE A20 OVARIAN TUMOUR (OTU) DOMAIN


Overview

The NF-kappaB (nuclear factor kappaB) regulator A20 antagonises IKK [IkappaB (inhibitor of kappaB) kinase] activation by modulating Lys63-linked polyubiquitination of cytokine-receptor-associated factors including TRAF2/6 (tumour-necrosis-factor-receptor-associated factor 2/6) and RIP1 (receptor-interacting protein 1). In the present paper we describe the crystal structure of the N-terminal OTU (ovarian tumour) deubiquitinase domain of A20, which differs from other deubiquitinases but shares the minimal catalytic core with otubain-2. Analysis of conserved surface regions allows prediction of ubiquitin-binding sites for the proximal and distal ubiquitin molecules. Structural and biochemical analysis suggests a novel architecture of the catalytic triad, which might be present in a subset of OTU domains including Cezanne and TRABID (TRAF-binding domain). Biochemical analysis shows a preference of the isolated A20 OTU domain for Lys48-linked tetraubiquitin in vitro suggesting that additional specificity factors might be required for the physiological function of A20 in cells.

About this Structure

2VFJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the A20 OTU domain and mechanistic insights into deubiquitination., Komander D, Barford D, Biochem J. 2008 Jan 1;409(1):77-85. PMID:17961127

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