2vh5

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[[Image:2vh5.jpg|left|200px]]<br /><applet load="2vh5" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2vh5.jpg|left|200px]]
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caption="2vh5, resolution 2.70&Aring;" />
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'''CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX'''<br />
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{{Structure
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|PDB= 2vh5 |SIZE=350|CAPTION= <scene name='initialview01'>2vh5</scene>, resolution 2.70&Aring;
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|SITE= <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+R'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+R'>AC2</scene> and <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+H'>AC3</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2VH5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+R'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+R'>AC2</scene> and <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+H'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VH5 OCA].
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2VH5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VH5 OCA].
==Reference==
==Reference==
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Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds., Tanaka T, Rabbitts TH, J Mol Biol. 2008 Feb 22;376(3):749-57. Epub 2007 Dec 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18187153 18187153]
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Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds., Tanaka T, Rabbitts TH, J Mol Biol. 2008 Feb 22;376(3):749-57. Epub 2007 Dec 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18187153 18187153]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: signaling protein/immune system]]
[[Category: signaling protein/immune system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:56:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:46:33 2008''

Revision as of 16:46, 20 March 2008

Image:2vh5.jpg


PDB ID 2vh5

Drag the structure with the mouse to rotate
, resolution 2.70Å
Sites: , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX


Overview

Intracellular antibody fragments that interfere with molecular interactions inside cells are valuable in investigation of interactomes and in therapeutics, but their application demands that they function in the reducing cellular milieu. We show here a 2.7-A crystal structure of intracellular antibody folds based on scaffolds developed from intracellular antibody capture technology, and we reveal that there is no structural or functional difference with or without the intra-domain disulfide bond of the variable domain of heavy chain or the variable domain of light chain. The data indicate that, in the reducing in vivo environment, the absence of the intra-domain disulfide bond is not an impediment to correction of antibody folding or to interaction with antigen. Thus, the structural constraints for in-cell function are intrinsic to variable single-domain framework sequences, providing a generic scaffold for isolation of functional intracellular antibody single domains.

About this Structure

2VH5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds., Tanaka T, Rabbitts TH, J Mol Biol. 2008 Feb 22;376(3):749-57. Epub 2007 Dec 4. PMID:18187153

Page seeded by OCA on Thu Mar 20 18:46:33 2008

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