4u7a
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==The carboxy-terminal domain of Erb1 is a seven-bladed beta-propeller that binds RNA.== |
| + | <StructureSection load='4u7a' size='340' side='right' caption='[[4u7a]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4u7a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U7A FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u7a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u7a RCSB], [http://www.ebi.ac.uk/pdbsum/4u7a PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ERB1_YEAST ERB1_YEAST]] Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[HAMAP-Rule:MF_03027]<ref>PMID:11522832</ref> <ref>PMID:18448671</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Erb1 (Eukaryotic Ribosome Biogenesis 1) protein is essential for the maturation of the ribosomal 60S subunit. Functional studies in yeast and mammalian cells showed that altogether with Nop7 and Ytm1 it forms a stable subcomplex called PeBoW that is crucial for a correct rRNA processing. The exact function of the protein within the process remains unknown. The N-terminal region of the protein includes a well conserved region shown to be involved in PeBoW complex formation whereas the carboxy-terminal half was predicted to contain seven WD40 repeats. This first structural report on Erb1 from yeast describes the architecture of a seven-bladed beta-propeller domain that revealed a characteristic extra motif formed by two alpha-helices and a beta-strand that insert within the second WD repeat. We performed analysis of molecular surface and crystal packing, together with multiple sequence alignment and comparison of the structure with other beta-propellers, in order to identify areas that are more likely to mediate protein-protein interactions. The abundance of many positively charged residues on the surface of the domain led us to investigate whether the propeller of Erb1 might be involved in RNA binding. Three independent assays confirmed that the protein interacted in vitro with polyuridilic acid (polyU), thus suggesting a possible role of the domain in rRNA rearrangement during ribosome biogenesis. | ||
| - | The | + | The Carboxy-Terminal Domain of Erb1 Is a Seven-Bladed ss-Propeller that Binds RNA.,Marcin W, Neira JL, Bravo J PLoS One. 2015 Apr 16;10(4):e0123463. doi: 10.1371/journal.pone.0123463., eCollection 2015. PMID:25880847<ref>PMID:25880847</ref> |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bravo, J]] | ||
[[Category: Wegrecki, M]] | [[Category: Wegrecki, M]] | ||
| - | [[Category: | + | [[Category: Beta-propeller]] |
| + | [[Category: Protein binding]] | ||
| + | [[Category: Ribosome biogenesis]] | ||
| + | [[Category: Rrna binding]] | ||
| + | [[Category: Wd40]] | ||
Revision as of 11:35, 30 April 2015
The carboxy-terminal domain of Erb1 is a seven-bladed beta-propeller that binds RNA.
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