2yqh

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[[Image:2yqh.gif|left|200px]]<br /><applet load="2yqh" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2yqh.gif|left|200px]]
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caption="2yqh, resolution 2.30&Aring;" />
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'''Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form'''<br />
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{{Structure
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|PDB= 2yqh |SIZE=350|CAPTION= <scene name='initialview01'>2yqh</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GN1:2-(ACETYLAMINO)-2-DEOXY-1-O-PHOSPHONO-ALPHA-D-GLUCOPYRANOSE'>GN1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_diphosphorylase UDP-N-acetylglucosamine diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.23 2.7.7.23]
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|GENE=
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}}
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'''Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2YQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=GN1:'>GN1</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_diphosphorylase UDP-N-acetylglucosamine diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.23 2.7.7.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YQH OCA].
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2YQH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YQH OCA].
==Reference==
==Reference==
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Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism., Maruyama D, Nishitani Y, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2007 Jun 8;282(23):17221-30. Epub 2007 Mar 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17392279 17392279]
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Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism., Maruyama D, Nishitani Y, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2007 Jun 8;282(23):17221-30. Epub 2007 Mar 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17392279 17392279]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: MG]]
[[Category: MG]]
[[Category: SO4]]
[[Category: SO4]]
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[[Category: candida albicans]]
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[[Category: candida albican]]
[[Category: crystal structure]]
[[Category: crystal structure]]
[[Category: n-acetylglucosamine]]
[[Category: n-acetylglucosamine]]
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[[Category: uridine-diphospho-n-acetylglucosamine]]
[[Category: uridine-diphospho-n-acetylglucosamine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:57:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:48:25 2008''

Revision as of 16:48, 20 March 2008

Image:2yqh.gif


PDB ID 2yqh

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands: , , and
Activity: UDP-N-acetylglucosamine diphosphorylase, with EC number 2.7.7.23
Coordinates: save as pdb, mmCIF, xml



Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans, in the substrate-binding form


Overview

Uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc) is a precursor of the bacterial and fungal cell wall. It is also used in a component of N-linked glycosylation and the glycosylphosphoinositol anchor of eukaryotic proteins. It is synthesized from N-acetylglucosamine-1-phosphate (GlcNAc-1-P) and uridine-5'-triphosphate (UTP) by UDP-GlcNAc pyrophosphorylase (UAP). This is an S(N)2 reaction; the non-esterified oxygen atom of the GlcNAc-1-P phosphate group attacks the alpha-phosphate group of UTP. We determined crystal structures of UAP from Candida albicans (CaUAP1) without any ligands and also complexed with its substrate or with its product. The series of structures in different forms shows the induced fit movements of CaUAP1. Three loops approaching the ligand molecule close the active site when ligand is bound. In addition, Lys-421, instead of the metal ion in prokaryotic UAPs, is coordinated by both phosphate groups of UDP-Glc-NAc and acts as a cofactor. However, a magnesium ion enhances the enzymatic activity of CaUAP1, and thus we propose that the magnesium ion increases the affinity between UTP and the enzyme by coordinating to the alpha- and gamma-phosphate group of UTP.

About this Structure

2YQH is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism., Maruyama D, Nishitani Y, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2007 Jun 8;282(23):17221-30. Epub 2007 Mar 28. PMID:17392279

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