Regulator of G protein signaling
From Proteopedia
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== Function == | == Function == | ||
| + | The structure 1AGR has in total 4 chains. These are represented by 2 sequence-unique entities. 1AGR is a tetramer structure of two identical duplicate crystal complex of RGS4- Gα<sub>i</sub>1 (tetramer excess stability of crystal structure). | ||
| + | Monomer structure of RGS4 in cartoon diagram: The RGS4 domain corresponds to an array of nine α-helices that fold into two small subdomains. The terminal subdomain contains the N and C termini of the box and is formed by α1, α2, α3, α8, and α9. Helices α1 and α9 lie in antiparallel orientation, juxtaposing the N and C termini of the box. The larger bundle subdomain, formed by α4, α5, α6, and α7, is a classic right-handed, antiparallel four-helix bundle. Both subdomains are required for GAP activity. | ||
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| + | Gα<sub>i</sub>1 subunits adopt a conserved fold composed of α helical domain (light gray cartoon), a helical domain of six α helices and a GTPase domain (dark gray cartoon).The GTPase domain hydrolyzes GTP and provides most of Gα's binding surfaces for Gβγ, receptors, effectors and RGS proteins. The GTPase domain contains three flexible regions designated switch-I<scene name='70/701447/Gi-s1/2'>switch-I</scene>,<scene name='70/701447/Gi-s2/2'>Switch-II</scene> and -III that change conformation in response to GTP binding and hydrolysis. The three switch regions of Gα<sub>i</sub>1: residues 176–184, 201–215, and 233–241, respectively (red cartoon). GDP–Mg+2, bound in the active site of Gα<sub>i</sub>1 is shown as a ball-and-stick model. For clarity, AlF4 is omitted from the figure. | ||
| - | == Relevance == | ||
== Structural highlights == | == Structural highlights == | ||
| - | <scene name='70/701447/Gi-s1/2'>Gi- Switch region 1 residues</scene> | ||
| - | <scene name='70/701447/Gi-s2/2'>Gi- Switch region 2 residues</scene> | ||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
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<references/> | <references/> | ||
Revision as of 13:21, 5 May 2015
Regulator of G protein signaling (RGS) interactions with G proteins – RGS4-Gαi as a model structure.
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References
Proteopedia Page Contributors and Editors (what is this?)
Ali Asli, Denise Salem, Michal Harel, Joel L. Sussman, Jaime Prilusky
