2yz1
From Proteopedia
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| - | [[Image:2yz1.jpg|left|200px]] | + | [[Image:2yz1.jpg|left|200px]] |
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| - | '''Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha''' | + | {{Structure |
| + | |PDB= 2yz1 |SIZE=350|CAPTION= <scene name='initialview01'>2yz1</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Shps1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2YZ1 is a [ | + | 2YZ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YZ1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47., Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A, J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:[http:// | + | Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47., Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A, J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18045614 18045614] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: immunoglobulin domain]] | [[Category: immunoglobulin domain]] | ||
[[Category: membrane]] | [[Category: membrane]] | ||
| - | [[Category: national project on protein structural and functional | + | [[Category: national project on protein structural and functional analyse]] |
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
[[Category: polymorphism]] | [[Category: polymorphism]] | ||
[[Category: sh3-binding]] | [[Category: sh3-binding]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:50:30 2008'' |
Revision as of 16:50, 20 March 2008
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| , resolution 1.40Å | |||||||
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| Gene: | Shps1 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha
Overview
SRC homology 2 domain-containing protein tyrosine phosphatase substrate 1 (SHPS-1 or SIRP alpha/BIT) is an immunoglobulin (Ig) superfamily transmembrane receptor and a member of the signal regulatory protein (SIRP) family involved in cell-cell interaction. SHPS-1 binds to its ligand CD47 to relay an inhibitory signal for cellular responses, whereas SIRPbeta, an activating member of the same family, does not bind to CD47 despite sharing a highly homologous ligand-binding domain with SHPS-1. To address the molecular basis for specific CD47 recognition by SHPS-1, we present the crystal structure of the ligand-binding domain of murine SHPS-1 (mSHPS-1). Folding topology revealed that mSHPS-1 adopts an I2-set Ig fold, but its overall structure resembles IgV domains of antigen receptors, although it has an extended loop structure (C'E loop), which forms a dimer interface in the crystal. Site-directed mutagenesis studies of mSHPS-1 identified critical residues for CD47 binding including sites in the C'E loop and regions corresponding to complementarity-determining regions of antigen receptors. The structural and functional features of mSHPS-1 are consistent with the human SHPS-1 structure except that human SHPS-1 has an additional beta-strand D. These results suggest that the variable complementarity-determining region-like loop structures in the binding surface of SHPS-1 are generally required for ligand recognition in a manner similar to that of antigen receptors, which may explain the diverse ligand-binding specificities of SIRP family receptors.
About this Structure
2YZ1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47., Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A, J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:18045614
Page seeded by OCA on Thu Mar 20 18:50:30 2008
Categories: Mus musculus | Single protein | Nakaishi, A. | Alternative splicing | Beta-sandwich | Glycoprotein | Immune system | Immunoglobulin domain | Membrane | National project on protein structural and functional analyse | Nppsfa | Phosphorylation | Polymorphism | Sh3-binding | Structural genomic | Transmembrane
