User:Wayne Decatur/Gal 4
From Proteopedia
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===DNA Recognition by Gal4p=== | ===DNA Recognition by Gal4p=== | ||
Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose. | Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose. | ||
| + | Gal4p is an 881-amino-acid protein with a Zn cluster-type DNA-binding domain, a linker domain, a dimerization domain, and two acidic activation domains. | ||
The structure of The protein binds as a dimer to a symmetrical 17-base-pair sequence. Specifically, the consensus Gal4-binding site is a 17-mer of sequence 5′-CGG-N11-CCG-3′. | The structure of The protein binds as a dimer to a symmetrical 17-base-pair sequence. Specifically, the consensus Gal4-binding site is a 17-mer of sequence 5′-CGG-N11-CCG-3′. | ||
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| + | Pioneering studies on Gal4p from the Ptashne laboratory shaped our understanding of transcriptional activation in eukaryotes and Gal4p derivatives, along with the congnate promoters, have been used as tools in yeast for many years. | ||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:1557122</ref><references group="xtra"/> | <ref group="xtra">PMID:1557122</ref><references group="xtra"/> | ||
Revision as of 15:39, 12 May 2015
Gal4p is a transcriptional activator in Saccharomyces cerevisiae that regulates the expression of genes to coordinate the response to the carbon source galactose. Structures
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DNA Recognition by Gal4p
Gal4p is a transcriptional activator in Saccharomyces cerevisiae that regulates the expression of genes to coordinate the response to the carbon source galactose. Gal4p is an 881-amino-acid protein with a Zn cluster-type DNA-binding domain, a linker domain, a dimerization domain, and two acidic activation domains. The structure of The protein binds as a dimer to a symmetrical 17-base-pair sequence. Specifically, the consensus Gal4-binding site is a 17-mer of sequence 5′-CGG-N11-CCG-3′.
Pioneering studies on Gal4p from the Ptashne laboratory shaped our understanding of transcriptional activation in eukaryotes and Gal4p derivatives, along with the congnate promoters, have been used as tools in yeast for many years.
Reference
- Marmorstein R, Carey M, Ptashne M, Harrison SC. DNA recognition by GAL4: structure of a protein-DNA complex. Nature. 1992 Apr 2;356(6368):408-14. PMID:1557122 doi:http://dx.doi.org/10.1038/356408a0
