2z2r
From Proteopedia
(New page: 200px<br /><applet load="2z2r" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z2r, resolution 3.2Å" /> '''Nucleosome assembly p...) |
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| - | [[Image:2z2r.jpg|left|200px]] | + | [[Image:2z2r.jpg|left|200px]] |
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| - | '''Nucleosome assembly proteins I (NAP-1, 74-365)''' | + | {{Structure |
| + | |PDB= 2z2r |SIZE=350|CAPTION= <scene name='initialview01'>2z2r</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= NAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
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| + | '''Nucleosome assembly proteins I (NAP-1, 74-365)''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2Z2R is a [ | + | 2Z2R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z2R OCA]. |
==Reference== | ==Reference== | ||
| - | A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1., Park YJ, McBryant SJ, Luger K, J Mol Biol. 2008 Jan 25;375(4):1076-85. Epub 2007 Nov 19. PMID:[http:// | + | A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1., Park YJ, McBryant SJ, Luger K, J Mol Biol. 2008 Jan 25;375(4):1076-85. Epub 2007 Nov 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18068721 18068721] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nucleosome assembly protein 1 (nap1)]] | [[Category: nucleosome assembly protein 1 (nap1)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:51:36 2008'' |
Revision as of 16:51, 20 March 2008
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| , resolution 3.2Å | |||||||
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| Gene: | NAP1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nucleosome assembly proteins I (NAP-1, 74-365)
Overview
The histone chaperone nucleosome assembly protein 1 (NAP1) is implicated in histone shuttling as well as nucleosome assembly and disassembly. Under physiological conditions, NAP1 dimers exist in a mixture of various high-molecular-weight oligomers whose size may be regulated by the cell cycle-dependent concentration of NAP1. Both the functional and structural significance of the observed oligomers are unknown. We have resolved the molecular mechanism by which yeast NAP1 (yNAP1) dimers oligomerize by applying x-ray crystallographic, hydrodynamic, and functional approaches. We found that an extended beta-hairpin that protrudes from the compact core of the yNAP1 dimer forms a stable beta-sheet with beta-hairpins of neighboring yNAP1 dimers. Disruption of the beta-hairpin (whose sequence is conserved among NAP1 proteins in various species) by the replacement of one or more amino acids with proline results in complete loss of yNAP1 dimer oligomerization. The in vitro functions of yNAP1 remain unaffected by the mutations. We have thus identified a conserved structural feature of NAP1 whose function, in addition to presenting the nuclear localization sequence, appears to be the formation of higher-order oligomers.
About this Structure
2Z2R is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1., Park YJ, McBryant SJ, Luger K, J Mol Biol. 2008 Jan 25;375(4):1076-85. Epub 2007 Nov 19. PMID:18068721
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