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4qca
From Proteopedia
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| - | ''' | + | ==Crystal structure of Vaccinia virus uracil-DNA glycosylase mutant R167AD4== |
| + | <StructureSection load='4qca' size='340' side='right' caption='[[4qca]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4qca]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QCA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dof|4dof]], [[4dog|4dog]], [[4lzb|4lzb]], [[4irb|4irb]], [[4qc9|4qc9]], [[4qcb|4qcb]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qca RCSB], [http://www.ebi.ac.uk/pdbsum/4qca PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/UNG_VACCA UNG_VACCA]] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Amino-acid residues located at a highly flexible area in the uracil DNA glycosylase of Vaccinia virus were mutated. In the crystal structure of wild-type D4 these residues lie at the dimer interface. Specifically, three mutants were generated: (i) residue Arg167 was replaced with an alanine (R167AD4), (ii) residues Glu171, Ser172 and Pro173 were substituted with three glycine residues (3GD4) and (iii) residues Glu171 and Ser172 were deleted (Delta171-172D4). Mutant proteins were expressed, purified and crystallized in order to investigate the effects of these mutations on the structure of the protein. | ||
| - | + | Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase.,Sartmatova D, Nash T, Schormann N, Nuth M, Ricciardi R, Banerjee S, Chattopadhyay D Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):295-301., doi: 10.1107/S1744309113002716. Epub 2013 Feb 23. PMID:23519808<ref>PMID:23519808</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Uracil-DNA glycosylase]] | ||
[[Category: Banerjee, S]] | [[Category: Banerjee, S]] | ||
| - | [[Category: | + | [[Category: Chattopadhyay, D]] |
[[Category: Nash, T]] | [[Category: Nash, T]] | ||
[[Category: Nuth, M]] | [[Category: Nuth, M]] | ||
| - | [[Category: | + | [[Category: Ricciardi, R]] |
| + | [[Category: Sartmatova, D]] | ||
| + | [[Category: Schormann, N]] | ||
| + | [[Category: A20]] | ||
| + | [[Category: Component of processivity factor]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Dna repair enzyme]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Poxvirus]] | ||
Revision as of 12:12, 13 May 2015
Crystal structure of Vaccinia virus uracil-DNA glycosylase mutant R167AD4
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