This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4uzb
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)== |
| + | <StructureSection load='4uzb' size='340' side='right' caption='[[4uzb]], [[Resolution|resolution]] 2.87Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4uzb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UZB FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uzc|4uzc]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uzb RCSB], [http://www.ebi.ac.uk/pdbsum/4uzb PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The latency-associated nuclear antigen (LANA) is the latent origin-binding protein and chromatin anchor of the Kaposi's sarcoma herpesvirus (KSHV/HHV-8) genome. Its C-terminal domain (CTD) binds sequence-specifically to the viral origin of replication, whereas the N-terminal domain links it to nucleosomes of cellular chromatin for long-term persistence in dividing host cells. Here, the crystallization and X-ray data acquisition of a mutant LANA CTD in complex with its wild-type target DNA LBS1 is described. This report describes the rational protein engineering for successful co-crystallization with DNA and X-ray diffraction data collection at room temperature on the high-brilliance third-generation synchrotron PETRA III at DESY, Germany. | ||
| - | + | Crystallization, room-temperature X-ray diffraction and preliminary analysis of Kaposi's sarcoma herpesvirus LANA bound to DNA.,Hellert J, Krausze J, Schulz TF, Luhrs T Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1570-4. doi:, 10.1107/S2053230X14019906. Epub 2014 Oct 25. PMID:25372834<ref>PMID:25372834</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Hellert, J]] | [[Category: Hellert, J]] | ||
| + | [[Category: Krausze, J]] | ||
[[Category: Luhrs, T]] | [[Category: Luhrs, T]] | ||
| - | [[Category: | + | [[Category: Cancer]] |
| + | [[Category: Dna-binding domain]] | ||
| + | [[Category: Gammaherpesvirus]] | ||
| + | [[Category: Hhv-8]] | ||
| + | [[Category: Multicentric castleman's disease]] | ||
| + | [[Category: Oligomerization domain]] | ||
| + | [[Category: Origin-binding domain]] | ||
| + | [[Category: Primary effusion lymphoma]] | ||
| + | [[Category: Rhadinovirus]] | ||
| + | [[Category: Tumor virus]] | ||
| + | [[Category: Viral protein-dna complex]] | ||
Revision as of 12:16, 13 May 2015
KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)
| |||||||||||
