4uzb

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'''Unreleased structure'''
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==KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)==
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<StructureSection load='4uzb' size='340' side='right' caption='[[4uzb]], [[Resolution|resolution]] 2.87&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4uzb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UZB FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uzc|4uzc]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uzb RCSB], [http://www.ebi.ac.uk/pdbsum/4uzb PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The latency-associated nuclear antigen (LANA) is the latent origin-binding protein and chromatin anchor of the Kaposi's sarcoma herpesvirus (KSHV/HHV-8) genome. Its C-terminal domain (CTD) binds sequence-specifically to the viral origin of replication, whereas the N-terminal domain links it to nucleosomes of cellular chromatin for long-term persistence in dividing host cells. Here, the crystallization and X-ray data acquisition of a mutant LANA CTD in complex with its wild-type target DNA LBS1 is described. This report describes the rational protein engineering for successful co-crystallization with DNA and X-ray diffraction data collection at room temperature on the high-brilliance third-generation synchrotron PETRA III at DESY, Germany.
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The entry 4uzb is ON HOLD until Paper Publication
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Crystallization, room-temperature X-ray diffraction and preliminary analysis of Kaposi's sarcoma herpesvirus LANA bound to DNA.,Hellert J, Krausze J, Schulz TF, Luhrs T Acta Crystallogr F Struct Biol Commun. 2014 Nov;70(Pt 11):1570-4. doi:, 10.1107/S2053230X14019906. Epub 2014 Oct 25. PMID:25372834<ref>PMID:25372834</ref>
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Authors: Hellert, J., Krausze, J., Luhrs, T.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hellert, J]]
[[Category: Hellert, J]]
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[[Category: Krausze, J]]
[[Category: Luhrs, T]]
[[Category: Luhrs, T]]
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[[Category: Krausze, J]]
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[[Category: Cancer]]
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[[Category: Dna-binding domain]]
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[[Category: Gammaherpesvirus]]
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[[Category: Hhv-8]]
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[[Category: Multicentric castleman's disease]]
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[[Category: Oligomerization domain]]
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[[Category: Origin-binding domain]]
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[[Category: Primary effusion lymphoma]]
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[[Category: Rhadinovirus]]
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[[Category: Tumor virus]]
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[[Category: Viral protein-dna complex]]

Revision as of 12:16, 13 May 2015

KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)

4uzb, resolution 2.87Å

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