2zai
From Proteopedia
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| - | [[Image:2zai.jpg|left|200px]] | + | [[Image:2zai.jpg|left|200px]] |
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| - | '''Crystal structure of the soluble domain of STT3 from P. furiosus''' | + | {{Structure |
| + | |PDB= 2zai |SIZE=350|CAPTION= <scene name='initialview01'>2zai</scene>, resolution 2.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PF0156 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the soluble domain of STT3 from P. furiosus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ZAI is a [ | + | 2ZAI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAI OCA]. |
==Reference== | ==Reference== | ||
| - | Structure-guided identification of a new catalytic motif of oligosaccharyltransferase., Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D, EMBO J. 2008 Jan 9;27(1):234-43. Epub 2007 Nov 29. PMID:[http:// | + | Structure-guided identification of a new catalytic motif of oligosaccharyltransferase., Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D, EMBO J. 2008 Jan 9;27(1):234-43. Epub 2007 Nov 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18046457 18046457] |
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:20 2008'' |
Revision as of 16:53, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Ligands: | and | ||||||
| Gene: | PF0156 (Pyrococcus furiosus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the soluble domain of STT3 from P. furiosus
Overview
Asn-glycosylation is widespread not only in eukaryotes but also in archaea and some eubacteria. Oligosaccharyltransferase (OST) catalyzes the co-translational transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. Here, we report that a thermophilic archaeon, Pyrococcus furiosus OST is composed of the STT3 protein alone, and catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr/Ser-motif-dependent manner. We also determined the 2.7-A resolution crystal structure of the C-terminal soluble domain of Pyrococcus STT3. The structure-based multiple sequence alignment revealed a new motif, DxxK, which is adjacent to the well-conserved WWDYG motif in the tertiary structure. The mutagenesis of the DK motif residues in yeast STT3 revealed the essential role of the motif in the catalytic activity. The function of this motif may be related to the binding of the pyrophosphate group of lipid-linked oligosaccharide donors through a transiently bound cation. Our structure provides the first structural insights into the formation of the oligosaccharide-asparagine bond.
About this Structure
2ZAI is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Structure-guided identification of a new catalytic motif of oligosaccharyltransferase., Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D, EMBO J. 2008 Jan 9;27(1):234-43. Epub 2007 Nov 29. PMID:18046457
Page seeded by OCA on Thu Mar 20 18:53:20 2008
