2zcf
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2zcf.gif|left|200px]] | + | [[Image:2zcf.gif|left|200px]] |
| - | + | ||
| - | '''Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771''' | + | {{Structure |
| + | |PDB= 2zcf |SIZE=350|CAPTION= <scene name='initialview01'>2zcf</scene>, resolution 1.43Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | ||
| + | |GENE= nthA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]), nthB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1833 Rhodococcus erythropolis]) | ||
| + | }} | ||
| + | |||
| + | '''Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2ZCF is a [ | + | 2ZCF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCF OCA]. |
==Reference== | ==Reference== | ||
| - | Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:[http:// | + | Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16636455 16636455] |
[[Category: Nitrile hydratase]] | [[Category: Nitrile hydratase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 33: | Line 42: | ||
[[Category: lyase]] | [[Category: lyase]] | ||
[[Category: metal-binding]] | [[Category: metal-binding]] | ||
| - | [[Category: national project on protein structural and functional | + | [[Category: national project on protein structural and functional analyse]] |
[[Category: nitrile]] | [[Category: nitrile]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
| Line 41: | Line 50: | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:43 2008'' |
Revision as of 16:53, 20 March 2008
| |||||||
| , resolution 1.43Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | nthA (Rhodococcus erythropolis), nthB (Rhodococcus erythropolis) | ||||||
| Activity: | Nitrile hydratase, with EC number 4.2.1.84 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mutational study on Alpha-Gln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771
Overview
Nitrile hydratase (NHase) from Rhodococcus sp. N771 is a non-heme iron enzyme having post-translationally modified cysteine ligands, alphaCys112-SO2H and alphaCys114-SOH. We replaced alphaGln90, which is conserved in all known NHases and involved in the hydrogen-bond network around the catalytic center, with glutamic acid or asparagine. The kcat of alphaQ90E and alphaQ90N mutants decreased to 24% and 5% that of wild type respectively, but the effect of mutations on Km was not very significant. In both mutants, the alphaCys114-SOH modification appeared to be responsible for the catalysis as in native NHase. We crystallized the nitrosylated alphaQ90N mutant and determined its structure at a resolution of 1.43 A. The structure was basically identical to that of native nitrosylated NHase except for the mutated site and its vicinity. The structural difference between native and alphaQ90N mutant NHases suggested the importance of the hydrogen bond networks between alphaGln90 and the iron center for the catalytic activity.
About this Structure
2ZCF is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.
Reference
Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771., Takarada H, Kawano Y, Hashimoto K, Nakayama H, Ueda S, Yohda M, Kamiya N, Dohmae N, Maeda M, Odaka M, Biosci Biotechnol Biochem. 2006 Apr;70(4):881-9. PMID:16636455
Page seeded by OCA on Thu Mar 20 18:53:43 2008
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Hashimoto, K. | Kamiya, N. | Kawano, Y. | Maeda, M. | Nakayama, H. | Odaka, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Takarada, H. | Ueda, S. | Yohda, M. | FE | MG | Cysteine-sulfenic acid | Cysteine-sulfinic acid | Hydration | Iron | Lyase | Metal-binding | National project on protein structural and functional analyse | Nitrile | Nppsfa | Oxidation | Photo-activation | Photo-reactive | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
