2zg5

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[[Image:2zg5.jpg|left|200px]]<br /><applet load="2zg5" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2zg5.jpg|left|200px]]
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caption="2zg5, resolution 2.26&Aring;" />
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'''Crystal structure of LysN, alpha-aminoadipate aminotransferase (Leucine complex), from Thermus thermophilus HB27'''<br />
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{{Structure
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|PDB= 2zg5 |SIZE=350|CAPTION= <scene name='initialview01'>2zg5</scene>, resolution 2.26&Aring;
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|SITE= <scene name='pdbsite=AC1:LEU+Binding+Site+For+Residue+A+398'>AC1</scene>, <scene name='pdbsite=AC2:LEU+Binding+Site+For+Residue+C+398'>AC2</scene> and <scene name='pdbsite=AC3:LEU+Binding+Site+For+Residue+E+398'>AC3</scene>
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|LIGAND= <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/2-aminoadipate_transaminase 2-aminoadipate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.39 2.6.1.39]
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|GENE= lysN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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}}
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'''Crystal structure of LysN, alpha-aminoadipate aminotransferase (Leucine complex), from Thermus thermophilus HB27'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ZG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=LEU:'>LEU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 2DTV. Active as [http://en.wikipedia.org/wiki/2-aminoadipate_transaminase 2-aminoadipate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.39 2.6.1.39] Known structural/functional Sites: <scene name='pdbsite=AC1:LEU+Binding+Site+For+Residue+A+398'>AC1</scene>, <scene name='pdbsite=AC2:LEU+Binding+Site+For+Residue+C+398'>AC2</scene> and <scene name='pdbsite=AC3:LEU+Binding+Site+For+Residue+E+398'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZG5 OCA].
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2ZG5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entry 2DTV. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZG5 OCA].
==Reference==
==Reference==
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alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus., Miyazaki T, Miyazaki J, Yamane H, Nishiyama M, Microbiology. 2004 Jul;150(Pt 7):2327-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15256574 15256574]
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alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus., Miyazaki T, Miyazaki J, Yamane H, Nishiyama M, Microbiology. 2004 Jul;150(Pt 7):2327-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15256574 15256574]
[[Category: 2-aminoadipate transaminase]]
[[Category: 2-aminoadipate transaminase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 5 13:26:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:10 2008''

Revision as of 16:54, 20 March 2008

Image:2zg5.jpg


PDB ID 2zg5

Drag the structure with the mouse to rotate
, resolution 2.26Å
Sites: , and
Ligands:
Gene: lysN (Thermus thermophilus)
Activity: 2-aminoadipate transaminase, with EC number 2.6.1.39
Coordinates: save as pdb, mmCIF, xml



Crystal structure of LysN, alpha-aminoadipate aminotransferase (Leucine complex), from Thermus thermophilus HB27


Overview

The extremely thermophilic bacterium Thermus thermophilus HB27 synthesizes lysine through alpha-aminoadipate (AAA). In this study, a T. thermophilus gene encoding the enzyme that catalyses transamination of AAA was cloned as a mammalian kynurenine/AAA aminotransferase (Kat2) gene homologue. A T. thermophilus mutant with disruption of the Kat2 homologue required a longer lag phase for growth and showed slower growth in minimal medium. Furthermore, addition of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2-oxoisocaproate, 2-oxoisovalerate and 2-oxo-3-methylvalerate, intermediates of leucine, valine and isoleucine biosyntheses, respectively, along with oxaloacetate, a compound in the TCA cycle, as an amino acceptor. These results suggest multiple roles of LysN in several cellular metabolic pathways including lysine and branched-chain amino acid biosyntheses.

About this Structure

2ZG5 is a Single protein structure of sequence from Thermus thermophilus. This structure supersedes the now removed PDB entry 2DTV. Full crystallographic information is available from OCA.

Reference

alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus., Miyazaki T, Miyazaki J, Yamane H, Nishiyama M, Microbiology. 2004 Jul;150(Pt 7):2327-34. PMID:15256574

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