2n0r
From Proteopedia
(Difference between revisions)
| Line 5: | Line 5: | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2n0r RCSB], [http://www.ebi.ac.uk/pdbsum/2n0r PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2n0r RCSB], [http://www.ebi.ac.uk/pdbsum/2n0r PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines-independent of their ability to crystallize- and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies. | ||
| + | |||
| + | RNA structure determination by solid-state NMR spectroscopy.,Marchanka A, Simon B, Althoff-Ospelt G, Carlomagno T Nat Commun. 2015 May 11;6:7024. doi: 10.1038/ncomms8024. PMID:25960310<ref>PMID:25960310</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 09:57, 27 May 2015
RNA structure determination by solid-state NMR spectroscopy
| |||||||||||
