352d

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[[Image:352d.gif|left|200px]]<br /><applet load="352d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:352d.gif|left|200px]]
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caption="352d, resolution 0.950&Aring;" />
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'''THE CRYSTAL STRUCTURE OF A PARALLEL-STRANDED PARALLEL-STRANDED GUANINE TETRAPLEX AT 0.95 ANGSTROM RESOLUTION'''<br />
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{{Structure
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|PDB= 352d |SIZE=350|CAPTION= <scene name='initialview01'>352d</scene>, resolution 0.950&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE CRYSTAL STRUCTURE OF A PARALLEL-STRANDED PARALLEL-STRANDED GUANINE TETRAPLEX AT 0.95 ANGSTROM RESOLUTION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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352D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=352D OCA].
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352D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=352D OCA].
==Reference==
==Reference==
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The crystal structure of a parallel-stranded guanine tetraplex at 0.95 A resolution., Phillips K, Dauter Z, Murchie AI, Lilley DM, Luisi B, J Mol Biol. 1997 Oct 17;273(1):171-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9367755 9367755]
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The crystal structure of a parallel-stranded guanine tetraplex at 0.95 A resolution., Phillips K, Dauter Z, Murchie AI, Lilley DM, Luisi B, J Mol Biol. 1997 Oct 17;273(1):171-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9367755 9367755]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Dauter, Z.]]
[[Category: Dauter, Z.]]
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[[Category: u-dna]]
[[Category: u-dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:53 2008''

Revision as of 16:54, 20 March 2008

Image:352d.gif


PDB ID 352d

Drag the structure with the mouse to rotate
, resolution 0.950Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF A PARALLEL-STRANDED PARALLEL-STRANDED GUANINE TETRAPLEX AT 0.95 ANGSTROM RESOLUTION


Overview

In both DNA and RNA, stretches of guanine bases can form stable four-stranded helices in the presence of sodium or potassium ions. Sequences with a propensity to form guanine tetraplexes have been found in chromosomal telomers, immunoglobulin switch regions, and recombination sites. We report the crystal structure at 0.95 A resolution of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) in the presence of sodium ions. The four strands form a right-handed helix that is stabilized by hydrogen-bonding tetrads of co-planar guanine bases. Well-resolved sodium ions are found between and, at defined points, within tetrad planes and are coordinated with the guanine O6 groups. Nine calcium ions have been identified, each with a well-defined hepta-coordinate hydration shell. Hydrogen-bonding water patterns are observed within the tetraplex's helical grooves and clustered about the phosphate groups. Water molecules in the groove may form a hydrogen bond with the O4', and may affect the stacking behavior of guanine. Two distinct stacking arrangements are noted for the guanine tetrads. The thymine bases do not contribute to the four-stranded conformation, but instead stack to stabilize the crystal lattice. We present evidence that the sugar conformation is strained and propose that this originates from forces that optimize guanine base stacking. Discrete conformational disorder is observed at several places in the phosphodiester backbone, which results from a simple crankshaft rotation that requires no net change in the sugar conformation.

About this Structure

352D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

The crystal structure of a parallel-stranded guanine tetraplex at 0.95 A resolution., Phillips K, Dauter Z, Murchie AI, Lilley DM, Luisi B, J Mol Biol. 1997 Oct 17;273(1):171-82. PMID:9367755

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