379d

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[[Image:379d.gif|left|200px]]<br /><applet load="379d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:379d.gif|left|200px]]
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caption="379d, resolution 3.100&Aring;" />
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'''THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE'''<br />
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{{Structure
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|PDB= 379d |SIZE=350|CAPTION= <scene name='initialview01'>379d</scene>, resolution 3.100&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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379D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA].
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379D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA].
==Reference==
==Reference==
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The structural basis of hammerhead ribozyme self-cleavage., Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG, Cell. 1998 Mar 6;92(5):665-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9506521 9506521]
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The structural basis of hammerhead ribozyme self-cleavage., Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG, Cell. 1998 Mar 6;92(5):665-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9506521 9506521]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Beigelman, L.]]
[[Category: Beigelman, L.]]
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[[Category: rna hammerhead ribozyme]]
[[Category: rna hammerhead ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:07 2008''

Revision as of 16:55, 20 March 2008

Image:379d.gif


PDB ID 379d

Drag the structure with the mouse to rotate
, resolution 3.100Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE


Overview

We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self-cleavage. Use of a 5'-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.

About this Structure

379D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

The structural basis of hammerhead ribozyme self-cleavage., Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG, Cell. 1998 Mar 6;92(5):665-73. PMID:9506521

Page seeded by OCA on Thu Mar 20 18:55:07 2008

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