3b31
From Proteopedia
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| - | [[Image:3b31.jpg|left|200px]] | + | [[Image:3b31.jpg|left|200px]] |
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| - | '''Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA''' | + | {{Structure |
| + | |PDB= 3b31 |SIZE=350|CAPTION= <scene name='initialview01'>3b31</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=IRI:IRIDIUM HEXAMMINE ION'>IRI</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3B31 is a [ | + | 3B31 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B31 OCA]. |
==Reference== | ==Reference== | ||
| - | tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http:// | + | tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157151 18157151] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Costantino, D A.]] | [[Category: Costantino, D A.]] | ||
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[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: hybrid state]] | [[Category: hybrid state]] | ||
| - | [[Category: | + | [[Category: ire]] |
[[Category: rna structure]] | [[Category: rna structure]] | ||
[[Category: translation initiation]] | [[Category: translation initiation]] | ||
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[[Category: trna mimicry]] | [[Category: trna mimicry]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:44 2008'' |
Revision as of 16:55, 20 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
Overview
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited high-resolution structural information. The intergenic region (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to begin to understand IRES function. Here we present the crystal structure of a Dicistroviridae IGR IRES domain that interacts with the ribosome's decoding groove. We find that this RNA domain precisely mimics the transfer RNA anticodon-messenger RNA codon interaction, and its modeled orientation on the ribosome helps explain translocation without peptide bond formation. When combined with a previous structure, this work completes the first high-resolution description of an IRES RNA and provides insight into how RNAs can manipulate complex biological machines.
About this Structure
3B31 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:18157151
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