3b3r

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[[Image:3b3r.jpg|left|200px]]<br /><applet load="3b3r" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:3b3r.jpg|left|200px]]
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caption="3b3r, resolution 0.980&Aring;" />
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'''Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)'''<br />
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{{Structure
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|PDB= 3b3r |SIZE=350|CAPTION= <scene name='initialview01'>3b3r</scene>, resolution 0.980&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FAE:FLAVIN-N7+PROTONATED-ADENINE+DINUCLEOTIDE'>FAE</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6]
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|GENE=
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}}
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'''Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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3B3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=FAE:'>FAE</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B3R OCA].
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3B3R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B3R OCA].
==Reference==
==Reference==
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Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18029419 18029419]
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Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18029419 18029419]
[[Category: Cholesterol oxidase]]
[[Category: Cholesterol oxidase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: steroid metabolism]]
[[Category: steroid metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:03:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:51 2008''

Revision as of 16:55, 20 March 2008

Image:3b3r.jpg


PDB ID 3b3r

Drag the structure with the mouse to rotate
, resolution 0.980Å
Ligands: , and
Activity: Cholesterol oxidase, with EC number 1.1.3.6
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)


Overview

Two high-resolution structures of a double mutant of bacterial cholesterol oxidase in the presence or absence of a ligand, glycerol, are presented, showing the trajectory of glycerol as it binds in a Michaelis complex-like position in the active site. A group of three aromatic residues forces the oxidized isoalloxazine moiety to bend along the N5-N10 axis as a response to the binding of glycerol in the active site. Movement of these aromatic residues is only observed in the glycerol-bound structure, indicating that some tuning of the FAD redox potential is caused by the formation of the Michaelis complex during regular catalysis. This structural study suggests a possible mechanism of substrate-assisted flavin activation, improves our understanding of the interplay between the enzyme, its flavin cofactor and its substrate, and is of use to the future design of effective cholesterol oxidase inhibitors.

About this Structure

3B3R is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

Reference

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419

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