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4x8q
From Proteopedia
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| - | ''' | + | ==X-ray crystal structure of AlkD2 from Streptococcus mutans== |
| + | <StructureSection load='4x8q' size='340' side='right' caption='[[4x8q]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4x8q]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X8Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x8q RCSB], [http://www.ebi.ac.uk/pdbsum/4x8q PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA glycosylases are important repair enzymes that eliminate a diverse array of aberrant nucleobases from the genomes of all organisms. Individual bacterial species often contain multiple paralogs of a particular glycosylase, yet the molecular and functional distinctions between these paralogs are not well understood. The recently discovered HEAT-like repeat (HLR) DNA glycosylases are distributed across all domains of life and are distinct in their specificity for cationic alkylpurines and mechanism of damage recognition. Here, we describe a number of phylogenetically diverse bacterial species with two orthologs of the HLR DNA glycosylase AlkD. One ortholog, which we designate AlkD2, is substantially less conserved. The crystal structure of Streptococcus mutans AlkD2 is remarkably similar to AlkD but lacks the only helix present in AlkD that penetrates the DNA minor groove. We show that AlkD2 possesses only weak DNA binding affinity and lacks alkylpurine excision activity. Mutational analysis of residues along this DNA binding helix in AlkD substantially reduced binding affinity for damaged DNA, for the first time revealing the importance of this structural motif for damage recognition by HLR glycosylases. | ||
| - | + | A New Family of HEAT-Like Repeat Proteins Lacking a Critical Substrate Recognition Motif Present in Related DNA Glycosylases.,Mullins EA, Shi R, Kotsch LA, Eichman BF PLoS One. 2015 May 15;10(5):e0127733. doi: 10.1371/journal.pone.0127733., eCollection 2015. PMID:25978435<ref>PMID:25978435</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: Eichman, B | + | __TOC__ |
| - | [[Category: Mullins, E | + | </StructureSection> |
| + | [[Category: Eichman, B F]] | ||
| + | [[Category: Mullins, E A]] | ||
[[Category: Shi, R]] | [[Category: Shi, R]] | ||
| + | [[Category: Alk motif]] | ||
| + | [[Category: Heat repeat]] | ||
| + | [[Category: Unknown function]] | ||
Revision as of 15:50, 27 May 2015
X-ray crystal structure of AlkD2 from Streptococcus mutans
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