User:Shai Biran/ SAT

From Proteopedia

< User:Shai Biran(Difference between revisions)
Jump to: navigation, search
(New page: ==Your Heading Here (maybe something like 'Structure')== <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> This is a default text for you...)
Current revision (06:34, 2 June 2015) (edit) (undo)
 
Line 1: Line 1:
-
==Your Heading Here (maybe something like 'Structure')==
+
== Example page for Green fluorescent protein ("GFP") ==
-
<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
+
<StructureSection load='1ema' size='300' frame='true' side='right' caption='GFP ([[1ema]])' scene=''>
-
This is a default text for your page '''Shai Biran/ SAT'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
+
[[Image:1ema.gif|thumb|left|450px|Green fluorescent protein (1ema)]]
-
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
+
-
== Function ==
+
Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria (PDB entry 1ema), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
-
== Disease ==
+
Exploring the Structure
 +
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The chromophore, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>
-
== Relevance ==
+
</StructureSection>
-
== Structural highlights ==
+
==References==
 +
<references/>
-
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
+
==Quiz==
-
 
+
<quiz display=sample>
-
</StructureSection>
+
{How many alpha helics are in this structure?}
-
== References ==
+
- One
-
<references/>
+
- None
 +
+ Eleven
 +
- Twelve
 +
</quiz>

Current revision

Example page for Green fluorescent protein ("GFP")

GFP (1ema)

Drag the structure with the mouse to rotate

References

  1. ↑ Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

Quiz

Points added for a correct answer:  
Points for a wrong answer:
Ignore the questions' coefficients:

1. How many alpha helics are in this structure?

One
None
Eleven
Twelve

Your score is 0 / 0

Proteopedia Page Contributors and Editors (what is this?)

Shai Biran

Retrieved from "http://52.214.119.220/wiki/index.php/User:Shai_Biran/_SAT"
Personal tools