3b71
From Proteopedia
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| - | [[Image:3b71.jpg|left|200px]] | + | [[Image:3b71.jpg|left|200px]] |
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| - | '''CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase''' | + | {{Structure |
| + | |PDB= 3b71 |SIZE=350|CAPTION= <scene name='initialview01'>3b71</scene>, resolution 2.82Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_protein-tyrosine_kinase Non-specific protein-tyrosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.2 2.7.10.2] | ||
| + | |GENE= PTK2, FAK, FAK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3B71 is a [ | + | 3B71 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B71 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the interaction between focal adhesion kinase and CD4., Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST, J Mol Biol. 2008 Feb 1;375(5):1320-8. Epub 2007 Nov 22. PMID:[http:// | + | Structural basis for the interaction between focal adhesion kinase and CD4., Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST, J Mol Biol. 2008 Feb 1;375(5):1320-8. Epub 2007 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18078954 18078954] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Non-specific protein-tyrosine kinase]] | [[Category: Non-specific protein-tyrosine kinase]] | ||
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[[Category: tyrosine-protein kinase]] | [[Category: tyrosine-protein kinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:56:38 2008'' |
Revision as of 16:56, 20 March 2008
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| , resolution 2.82Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PTK2, FAK, FAK1 (Homo sapiens) | ||||||
| Activity: | Non-specific protein-tyrosine kinase, with EC number 2.7.10.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Overview
Focal adhesion kinase (FAK) and CD4 fulfil vital functions in cellular signal transduction: FAK is a central component in integrin signalling, whereas CD4 plays essential roles in the immune defence. In T lymphocytes, FAK and CD4 localise to the same signalling complexes after stimulation by either the human immunodeficiency virus (HIV) gp120 glycoprotein or an antigen, suggesting the concerted action of FAK and CD4 in these cells. Using crystallography and microcalorimetry, we here show that the focal adhesion targeting (FAT) domain of FAK binds specifically to the CD4 endocytosis motif in vitro. This FAT-CD4 complex is structurally and thermodynamically similar to the one FAT forms with paxillin LD motifs. The CD4 binding site on FAT presents the same features as the established CD4 binding site on the HIV-1 Nef protein. The binding of FAT to CD4 is incompatible with the binding of Lck to CD4. We further show that HIV-1 gp120 triggers the association of CD4 with FAK in T cells, under conditions that are known to dissociate Lck from CD4. Our results suggest that the FAK-CD4 complex represents an alternative route for eliciting T-cell-specific signals and that it links gp120 engagement to distinctive T-cell signalling during HIV infection. In infected cells, HIV-1 Nef may displace FAK from CD4 to protect the cells from apoptosis.
About this Structure
3B71 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for the interaction between focal adhesion kinase and CD4., Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST, J Mol Biol. 2008 Feb 1;375(5):1320-8. Epub 2007 Nov 22. PMID:18078954
Page seeded by OCA on Thu Mar 20 18:56:38 2008
Categories: Homo sapiens | Non-specific protein-tyrosine kinase | Protein complex | Arold, S T. | Garron, M L. | Acetylation | Alternative splicing | Atp-binding | Cell junction | Four-helix bundle | Glycoprotein | Host-virus interaction | Immune response | Immunoglobulin domain | Kinase | Lipoprotein | Membrane | Nucleotide-binding | Palmitate | Phosphorylation | Polymorphism | Protein binding | Protein-protein complex | Transferase | Transmembrane | Tyrosine-protein kinase
