3b8p
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:3b8p.jpg|left|200px]] | + | [[Image:3b8p.jpg|left|200px]] |
| - | + | ||
| - | '''Fragment of WzzB, Polysaccharide Co-polymerase from Salmonella Typhimurium''' | + | {{Structure |
| + | |PDB= 3b8p |SIZE=350|CAPTION= <scene name='initialview01'>3b8p</scene>, resolution 3.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= wzzB, cld, rol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | }} | ||
| + | |||
| + | '''Fragment of WzzB, Polysaccharide Co-polymerase from Salmonella Typhimurium''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 3B8P is a [ | + | 3B8P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8P OCA]. |
==Reference== | ==Reference== | ||
| - | Bacterial polysaccharide co-polymerases share a common framework for control of polymer length., Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Fethiere J, Matte A, Cygler M, Nat Struct Mol Biol. 2008 Feb;15(2):130-8. Epub 2008 Jan 20. PMID:[http:// | + | Bacterial polysaccharide co-polymerases share a common framework for control of polymer length., Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Fethiere J, Matte A, Cygler M, Nat Struct Mol Biol. 2008 Feb;15(2):130-8. Epub 2008 Jan 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18204465 18204465] |
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: wzzb]] | [[Category: wzzb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:57:10 2008'' |
Revision as of 16:57, 20 March 2008
| |||||||
| , resolution 3.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | wzzB, cld, rol (Salmonella typhimurium) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Fragment of WzzB, Polysaccharide Co-polymerase from Salmonella Typhimurium
Overview
The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length distributions to surface polysaccharides. Despite very low sequence similarities, they have a common protomer structure with a long central alpha-helix extending 100 A into the periplasm. The protomers self-assemble into bell-shaped oligomers of variable sizes, with a large internal cavity. Electron microscopy shows that one of the full-length PCPs has a similar organization as that observed in the crystal for its periplasmic domain alone. Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery.
About this Structure
3B8P is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length., Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Fethiere J, Matte A, Cygler M, Nat Struct Mol Biol. 2008 Feb;15(2):130-8. Epub 2008 Jan 20. PMID:18204465
Page seeded by OCA on Thu Mar 20 18:57:10 2008
