3bd9
From Proteopedia
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| - | [[Image:3bd9.jpg|left|200px]] | + | [[Image:3bd9.jpg|left|200px]] |
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| - | '''human 3-O-sulfotransferase isoform 5 with bound PAP''' | + | {{Structure |
| + | |PDB= 3bd9 |SIZE=350|CAPTION= <scene name='initialview01'>3bd9</scene>, resolution 2.30Å | ||
| + | |SITE= <scene name='pdbsite=AC1:A3p+Binding+Site+For+Residue+A+347'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] | ||
| + | |GENE= HS3ST5, 3OST5, HS3OST5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''human 3-O-sulfotransferase isoform 5 with bound PAP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3BD9 is a [ | + | 3BD9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BD9 OCA]. |
==Reference== | ==Reference== | ||
| - | Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:[http:// | + | Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18223645 18223645] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:04 2008'' |
Revision as of 16:58, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Gene: | HS3ST5, 3OST5, HS3OST5 (Homo sapiens) | ||||||
| Activity: | [Heparan_sulfate-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number 2.8.2.23 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
human 3-O-sulfotransferase isoform 5 with bound PAP
Overview
The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.
About this Structure
3BD9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:18223645[[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]]
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