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3bff

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[[Image:3bff.jpg|left|200px]]<br /><applet load="3bff" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:3bff.jpg|left|200px]]
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caption="3bff, resolution 1.9&Aring;" />
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'''class A beta-lactamase SED-G238C complexed with faropenem'''<br />
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{{Structure
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|PDB= 3bff |SIZE=350|CAPTION= <scene name='initialview01'>3bff</scene>, resolution 1.9&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>, <scene name='pdbligand=FPM:'>FPM</scene> and <scene name='pdbligand=SFR:'>SFR</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]
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|GENE= bla-SED-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=67826 Citrobacter sedlakii])
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}}
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'''class A beta-lactamase SED-G238C complexed with faropenem'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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3BFF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii] with <scene name='pdbligand=SCN:'>SCN</scene>, <scene name='pdbligand=FPM:'>FPM</scene> and <scene name='pdbligand=SFR:'>SFR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].
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3BFF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].
==Reference==
==Reference==
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Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684905 14684905]
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Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684905 14684905]
[[Category: Beta-lactamase]]
[[Category: Beta-lactamase]]
[[Category: Citrobacter sedlakii]]
[[Category: Citrobacter sedlakii]]
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[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:05:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:30 2008''

Revision as of 16:58, 20 March 2008

Image:3bff.jpg


PDB ID 3bff

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: , and
Gene: bla-SED-1 (Citrobacter sedlakii)
Activity: Beta-lactamase, with EC number 3.5.2.6
Coordinates: save as pdb, mmCIF, xml



class A beta-lactamase SED-G238C complexed with faropenem


Overview

SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C.

About this Structure

3BFF is a Single protein structure of sequence from Citrobacter sedlakii. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:14684905

Page seeded by OCA on Thu Mar 20 18:58:30 2008

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