4rzl
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==DNA recognition domain of the cytosine modification-dependent restriction endonuclease LpnPI== |
| + | <StructureSection load='4rzl' size='340' side='right' caption='[[4rzl]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4rzl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RZL FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rzl RCSB], [http://www.ebi.ac.uk/pdbsum/4rzl PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The eukaryotic Set and Ring Associated (SRA) domains and structurally similar DNA recognition domains of prokaryotic cytosine modification-dependent restriction endonucleases recognize methylated, hydroxymethylated or glucosylated cytosine in various sequence contexts. Here, we report the apo-structure of the N-terminal SRA-like domain of the cytosine modification-dependent restriction enzyme LpnPI that recognizes modified cytosine in the 5'-C(mC)DG-3' target sequence (where mC is 5-methylcytosine or 5-hydroxymethylcytosine and D = A/T/G). Structure-guided mutational analysis revealed LpnPI residues involved in base-specific interactions and demonstrated binding site plasticity that allowed limited target sequence degeneracy. Furthermore, modular exchange of the LpnPI specificity loops by structural equivalents of related enzymes AspBHI and SgrTI altered sequence specificity of LpnPI. Taken together, our results pave the way for specificity engineering of the cytosine modification-dependent restriction enzymes. | ||
| - | + | Structure-guided sequence specificity engineering of the modification-dependent restriction endonuclease LpnPI.,Sasnauskas G, Zagorskaite E, Kauneckaite K, Tamulaitiene G, Siksnys V Nucleic Acids Res. 2015 May 22. pii: gkv548. PMID:26001968<ref>PMID:26001968</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Sasnauskas, G]] | [[Category: Sasnauskas, G]] | ||
[[Category: Siksnys, V]] | [[Category: Siksnys, V]] | ||
[[Category: Tamulaitiene, G]] | [[Category: Tamulaitiene, G]] | ||
| + | [[Category: Cytosine modification]] | ||
| + | [[Category: Dna binding]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Lpnpi]] | ||
| + | [[Category: Restriction endonuclease]] | ||
| + | [[Category: Sra domain]] | ||
Revision as of 14:00, 10 June 2015
DNA recognition domain of the cytosine modification-dependent restriction endonuclease LpnPI
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