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3bun
From Proteopedia
(New page: 200px<br /><applet load="3bun" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bun, resolution 2.000Å" /> '''Crystal structure o...) |
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| - | [[Image:3bun.jpg|left|200px]] | + | [[Image:3bun.jpg|left|200px]] |
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| - | '''Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4''' | + | {{Structure |
| + | |PDB= 3bun |SIZE=350|CAPTION= <scene name='initialview01'>3bun</scene>, resolution 2.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CBL, CBL2, RNF55 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3BUN is a [ | + | 3BUN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BUN OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates., Ng C, Jackson RA, Buschdorf JP, Sun Q, Guy GR, Sivaraman J, EMBO J. 2008 Feb 14;. PMID:[http:// | + | Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates., Ng C, Jackson RA, Buschdorf JP, Sun Q, Guy GR, Sivaraman J, EMBO J. 2008 Feb 14;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18273061 18273061] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:01:22 2008'' |
Revision as of 17:01, 20 March 2008
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| , resolution 2.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | CBL, CBL2, RNF55 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty4
Overview
The c-Cbl tyrosine kinase binding domain (Cbl-TKB), essentially an 'embedded' SH2 domain, has a critical role in targeting proteins for ubiquitination. To address how this domain can bind to disparate recognition mofits and to determine whether this results in variations in substrate-binding affinity, we compared crystal structures of the Cbl-TKB domain complexed with phosphorylated peptides of Sprouty2, Sprouty4, epidermal growth factor receptor, Syk, and c-Met receptors and validated the binding with point-mutational analyses using full-length proteins. An obligatory, intrapeptidyl H-bond between the phosphotyrosine and the conserved asparagine or adjacent arginine is essential for binding and orientates the peptide into a positively charged pocket on c-Cbl. Surprisingly, c-Met bound to Cbl in the reverse direction, which is unprecedented for SH2 domain binding. The necessity of this intrapeptidyl H-bond was confirmed with isothermal titration calorimetry experiments that also showed Sprouty2 to have the highest binding affinity to c-Cbl; this may enable the selective sequestration of c-Cbl from other target proteins.
About this Structure
3BUN is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates., Ng C, Jackson RA, Buschdorf JP, Sun Q, Guy GR, Sivaraman J, EMBO J. 2008 Feb 14;. PMID:18273061
Page seeded by OCA on Thu Mar 20 19:01:22 2008
Categories: Homo sapiens | Protein complex | Buschdorf, J P. | Guy, G R. | Jackson, R A. | Ng, C. | Sivaraman, J. | Sun, Q. | Alternative splicing | Calcium | Cbl | Complex | Cytoplasm | Developmental protein | Ligase | Ligase/signaling protein complex | Membrane | Metal-binding | Phosphoprotein | Proto-oncogene | Sh2 domain | Signal transduction | Tkb | Ubl conjugation pathway | Zinc | Zinc-finger
