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4x2h
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Sac3N peptide bound to Mex67:Mtr2== |
| + | <StructureSection load='4x2h' size='340' side='right' caption='[[4x2h]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4x2h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X2H FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x2h RCSB], [http://www.ebi.ac.uk/pdbsum/4x2h PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The TREX-2 complex integrates mRNA nuclear export into the gene expression pathway and is based on a Sac3 scaffold to which Thp1, Sem1, Sus1, and Cdc31 bind. TREX-2 also binds the mRNA nuclear export factor, Mex67:Mtr2, through the Sac3 N-terminal region (Sac3N). Here, we characterize Chaetomium thermophilum TREX-2, show that the in vitro reconstituted complex has an annular structure, and define the structural basis for interactions between Sac3, Sus1, Cdc31, and Mex67:Mtr2. Crystal structures show that the binding of C. thermophilum Sac3N to the Mex67 NTF2-like domain (Mex67NTF2L) is mediated primarily through phenylalanine residues present in a series of repeating sequence motifs that resemble those seen in many nucleoporins, and Mlp1 also binds Mex67:Mtr2 using a similar motif. Deletion of Sac3N generated growth and mRNA export defects in Saccharomyces cerevisiae, and we propose TREX-2 and Mlp1 function to facilitate export by concentrating mature messenger ribonucleoparticles at the nuclear pore entrance. | ||
| - | + | Structural Characterization of the Chaetomium thermophilum TREX-2 Complex and its Interaction with the mRNA Nuclear Export Factor Mex67:Mtr2.,Dimitrova L, Valkov E, Aibara S, Flemming D, McLaughlin SH, Hurt E, Stewart M Structure. 2015 Jun 3. pii: S0969-2126(15)00180-X. doi:, 10.1016/j.str.2015.05.002. PMID:26051714<ref>PMID:26051714</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Aibara, S]] | [[Category: Aibara, S]] | ||
| + | [[Category: Stewart, M]] | ||
[[Category: Valkov, E]] | [[Category: Valkov, E]] | ||
| - | [[Category: | + | [[Category: Mrna nuclear export]] |
| + | [[Category: Transcription]] | ||
| + | [[Category: Trex-2]] | ||
Revision as of 15:13, 17 June 2015
Sac3N peptide bound to Mex67:Mtr2
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