4zkd
From Proteopedia
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/SKI7_YEAST SKI7_YEAST]] Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded.<ref>PMID:10074137</ref> <ref>PMID:11027292</ref> <ref>PMID:11514438</ref> <ref>PMID:11532933</ref> <ref>PMID:11910110</ref> <ref>PMID:12769863</ref> <ref>PMID:12881429</ref> <ref>PMID:14671320</ref> <ref>PMID:15933721</ref> <ref>PMID:6371496</ref> | [[http://www.uniprot.org/uniprot/SKI7_YEAST SKI7_YEAST]] Represses the expression of non-poly(A) mRNAs like L-A or M viruses and is therefore involved in antiviral system. Mediates interactions via its N-terminus between the exosome and the SKI complex which operate in the 3'-to-5' mRNA-decay pathway. By interacting with NAM7, is also required for nonsense-mediated 3'-to-5' mRNA-decay (NMD). May recognize a stalled 80S ribosome at the 3'-end of a nonstop mRNA which leads to the recruitment of the exosome and SKI complexes to the mRNAs to be degraded.<ref>PMID:10074137</ref> <ref>PMID:11027292</ref> <ref>PMID:11514438</ref> <ref>PMID:11532933</ref> <ref>PMID:11910110</ref> <ref>PMID:12769863</ref> <ref>PMID:12881429</ref> <ref>PMID:14671320</ref> <ref>PMID:15933721</ref> <ref>PMID:6371496</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ski7 is a cofactor of the cytoplasmic exosome in budding yeast, functioning in both mRNA turnover and non-stop decay (NSD), a surveillance pathway that degrades faulty mRNAs lacking a stop codon. The C-terminal region of Ski7 (Ski7C) shares overall sequence similarity with the translational GTPase (trGTPase) Hbs1, but whether Ski7 has retained the properties of a trGTPase is unclear. Here, we report the high-resolution structures of Ski7C bound to either intact guanosine triphosphate (GTP) or guanosine diphosphate-Pi. The individual domains of Ski7C adopt the conformation characteristic of active trGTPases. Furthermore, the nucleotide-binding site of Ski7C shares similar features compared with active trGTPases, notably the presence of a characteristic monovalent cation. However, a suboptimal polar residue at the putative catalytic site and an unusual polar residue that interacts with the gamma-phosphate of GTP distinguish Ski7 from other trGTPases, suggesting it might function rather as a GTP-binding protein than as a GTP-hydrolyzing enzyme. | ||
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| + | Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases.,Kowalinski E, Schuller A, Green R, Conti E Structure. 2015 May 28. pii: S0969-2126(15)00177-X. doi:, 10.1016/j.str.2015.04.018. PMID:26051716<ref>PMID:26051716</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 06:45, 24 June 2015
Crystal structure of the S. cerevisiae Ski7 GTPase-like domain, bound to GDP and inorganic phosphate.
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Categories: Conti, E | Kowalinski, E | Gtp-binding protein | Gtpase | Hydrolase | Ngd | Ski | Translation
