3nla
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:3nla.jpg|left|200px]] | + | [[Image:3nla.jpg|left|200px]] |
| - | + | ||
| - | '''NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF ANTARCTIC EEL POUT ANTIFREEZE PROTEIN RD3, 40 STRUCTURES''' | + | {{Structure |
| + | |PDB= 3nla |SIZE=350|CAPTION= <scene name='initialview01'>3nla</scene> | ||
| + | |SITE= <scene name='pdbsite=1:Ice+Binding+Site'>1</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF ANTARCTIC EEL POUT ANTIFREEZE PROTEIN RD3, 40 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 3NLA is a [ | + | 3NLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lycodichthys_dearborni Lycodichthys dearborni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NLA OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the binding of a globular antifreeze protein to ice., Jia Z, DeLuca CI, Chao H, Davies PL, Nature. 1996 Nov 21;384(6606):285-8. PMID:[http:// | + | Structural basis for the binding of a globular antifreeze protein to ice., Jia Z, DeLuca CI, Chao H, Davies PL, Nature. 1996 Nov 21;384(6606):285-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8918883 8918883] |
[[Category: Lycodichthys dearborni]] | [[Category: Lycodichthys dearborni]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: thermal hysteresis protein]] | [[Category: thermal hysteresis protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:06:06 2008'' |
Revision as of 17:06, 20 March 2008
| |||||||
| Sites: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF ANTARCTIC EEL POUT ANTIFREEZE PROTEIN RD3, 40 STRUCTURES
Overview
Antifreeze proteins (AFPs) have the unique ability to adsorb to ice and inhibit its growth. Many organisms ranging from fish to bacteria use AFPs to retard freezing or lessen the damage incurred upon freezing and thawing. The ice-binding mechanism of the long linear alpha-helical type I AFPs has been attributed to their regularly spaced polar residues matching the ice lattice along a pyramidal plane. In contrast, it is not known how globular antifreeze proteins such as type III AFP that lack repeating ice-binding residues bind to ice. Here we report the 1.25 A crystal structure of recombinant type III AFP (QAE isoform) from eel pout (Macrozoarces americanus), which reveals a remarkably flat amphipathic ice-binding site where five hydrogen-bonding atoms match two ranks of oxygens on the [1010] ice prism plane in the <0001> direction, giving high ice-binding affinity and specificity. This binding site, substantiated by the structures and properties of several ice-binding site mutants, suggests that the AFP occupies a niche in the ice surface in which it covers the basal plane while binding to the prism face.
About this Structure
3NLA is a Single protein structure of sequence from Lycodichthys dearborni. Full crystallographic information is available from OCA.
Reference
Structural basis for the binding of a globular antifreeze protein to ice., Jia Z, DeLuca CI, Chao H, Davies PL, Nature. 1996 Nov 21;384(6606):285-8. PMID:8918883
Page seeded by OCA on Thu Mar 20 19:06:06 2008
