Beta2 adrenergic receptor-Gs protein complex

Introduction

G protein-coupled receptors (GPCRs) are a large family of protein receptors which have seven-transmembrane helices and are found over a large array on eukaryotic cells. These receptors take major part in a multitude of signal transduction pathways, including amongst others responses to hormones and neurotransmitters, sensing light, taste and smell, and many more. These receptors are also involved in many different types of diseases and are the target of almost 50% of current medical drugs. The Beta-2 Adrenergic Receptors are a type of GPCRs which are activated by catecholamine hormone ligands such as adrenaline (epinephrine). These receptors are responsible for many of the adrenaline related (“fight-or-flight”) responses and functions, and are used as a common model system for the GPCR family. GPCRs bind their ligand and overcome a conformational change which allows a Guanine nucleotide-binding protein (G protein) to detach from the cellular end of the receptor and start the different signal transduction pathways.

G proteins ^[1] are a family of proteins that act as molecular switches inside cells. Their activity is regulated by factors that control their ability to bind to and hydrolyze guanosine triphosphate (GTP) to guanosine diphosphate (GDP). When they are bound to GTP, they are 'on', and, when they are bound to GDP, they are 'off'. G proteins belong to the larger group of enzymes called GTPases ^[2]. G proteins appear either as monomeric small GTPases ^[3], or as heterotrimeric G protein complexes ^[4] which are made up of alpha (α), beta (β) and gamma (γ) subunits ^[5].

Since these receptors have seven transmembrane helices as well as inner and outer cell regions, they are very difficult to purify and crystalize. Some crystal structures have been determined for the inactive receptors as well as for the G proteins that they bind. PDB entry 3SN6 is the first structure of the full complex of the Beta 2 Adrenergic Receptor bound to Gs in their active state, and it provides the first high-resolution insight into the mechanism of signal transduction across the plasma membrane by a GPCR.

Complex structure

The overall structure shows the (dark blue) bound to an agonist (in spheres) along with a T4 lysozyme fused to its amino terminus in order to facilitate crystallization. The receptor interacts with Gαs (light blue). Gαs together with Gβ (light green) and Gγ (gold) constitute the heterotrimeric G protein Gs. A Gs-binding nanobody which also facilitates crystallization (pink) binds the G protein between the a and b subunits.

G-Protein-GPCR Intercations

The α5-helix of Gαs docks into a cavity formed on the intracellular side of the receptor by the opening of transmembrane helices 5 and 6. Within the transmembrane core, the interactions are primarily non-polar - involves packing of Tyr 391 of the α5-helix against Arg 131 of the conserved DRY sequence in TM3. Arg 131 also packs against Tyr 326 of the conserved NPxxY sequence in TM7. As the α5-helix exits the receptor it forms a network of polar interactions with TM5 and TM3. Receptor residues Thr 68 and Asp 130 interact with the ICL2 helix of the β2AR via Tyr 141, positioning the helix so that Phe 139 of the receptor a hydrophobic pocket on the G protein surface, thereby structurally linking receptor–G protein interactions with the highly conserved DRY motif of the β2AR.

G-Protein Activation Cycle

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The figure shows the G Protein cycle ^[6] - an extracellular agonist binding to the β2AR leads to of the cytoplasmic ends of transmembrane segments that enable the Gs heterotrimer to bind the receptor. GDP is released from the α subunit upon formation of β2AR–Gs complex. The GTP binds to the nucleotide-free α subunit resulting in dissociation of the α and βγ subunits from the receptor. The subunits regulate their respective effector proteins adenylyl cyclase (AC) and Ca2+ channels. The Gs heterotrimer reassembles from α and βγ subunits following hydrolysis of GTP to GDP in the α subunit.

G-Protein variability

The Gαs subunit consists of two domains, the Ras domain (αRas) and the α-helical domain (αAH). Both are involved in nucleotide binding. A big discovery made thanks to this structure is that the Gα subunit has large variability between its GTP bound (active) and nucleotide free states, where the αAH domain has a relative to the αRas domain.

See Also

• Adrenergic receptor
• Antibody
• Beta-2 Adrenergic Receptor
• G protein-coupled receptor
• GTP-binding protein
• Guanine nucleotide-binding protein
• Hormone
• Lysozyme 3D structures
• Nobel Prizes for 3D Molecular Structure
• Suggestions for new articles
• Transducin
• User:Wayne Decatur/UNH BCHEM833 Structural Analysis Workshop Session Fall 2012
• User:Wayne Decatur/UNH BCHEM833 Structural Proteomics Introductory Lecture Fall 2012
• 3SN6