417d
From Proteopedia
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| - | [[Image:417d.gif|left|200px]] | + | [[Image:417d.gif|left|200px]] |
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| - | '''A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE''' | + | {{Structure |
| + | |PDB= 417d |SIZE=350|CAPTION= <scene name='initialview01'>417d</scene>, resolution 1.500Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 417D is a [ | + | 417D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=417D OCA]. |
==Reference== | ==Reference== | ||
| - | A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:[http:// | + | A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9753550 9753550] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Brown, D M.]] | [[Category: Brown, D M.]] | ||
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[[Category: Moore, M H.]] | [[Category: Moore, M H.]] | ||
[[Category: Schuerman, M H.]] | [[Category: Schuerman, M H.]] | ||
| - | [[Category: p a base- | + | [[Category: p a base-pair]] |
[[Category: pyrimidine analogue]] | [[Category: pyrimidine analogue]] | ||
[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:00 2008'' |
Revision as of 17:08, 20 March 2008
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| , resolution 1.500Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE
Overview
The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are of the wobble type. This result appears inconsistent with other evidence from hybridisation and NMR studies of P-containing oligonucleotides, which suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation experiments over a range of solution pH values indicate that protonation of the P.A base-pairs is unlikely to be responsible for the anomalous behaviour. No specific crystal packing effects can be identfied as an explanation, and it is concluded that base stacking and other interactions between nucleotide residues in Z-DNA are responsible.
About this Structure
417D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:9753550
Page seeded by OCA on Thu Mar 20 19:08:00 2008
