439d

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[[Image:439d.gif|left|200px]]<br /><applet load="439d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:439d.gif|left|200px]]
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caption="439d, resolution 1.6&Aring;" />
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'''5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3''''<br />
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{{Structure
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|PDB= 439d |SIZE=350|CAPTION= <scene name='initialview01'>439d</scene>, resolution 1.6&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=BA:BARIUM ION'>BA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3''''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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439D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BA:'>BA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=439D OCA].
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439D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=439D OCA].
==Reference==
==Reference==
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Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11173467 11173467]
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Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173467 11173467]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Betzel, C.]]
[[Category: Betzel, C.]]
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[[Category: fragment of 5s rrna]]
[[Category: fragment of 5s rrna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:22 2008''

Revision as of 17:08, 20 March 2008


PDB ID 439d

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, resolution 1.6Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'


Overview

The structure of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) has been determined at 1.6 A resolution and refined to a final R factor of 18.3% (R(free) = 24.1%). The sequence of the RNA fragment resembles domain E of Thermus flavus 5S rRNA. A previously undescribed wobble-like G.C base-pair formation is found. Owing to the observed hydrogen-bond network, it is proposed that the cytosine is protonated at position N3. The unusual base-pair formation is presumably strained by intermolecular interactions. In this context, crystal packing and particular intermolecular contacts may have direct influence on the three-dimensional structure. Furthermore, this structure includes two G.U wobble base pairs in tandem conformation, with the purines forming a so-called 'cross-strand G stack'.

About this Structure

439D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:11173467

Page seeded by OCA on Thu Mar 20 19:08:22 2008

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