467d
From Proteopedia
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| - | [[Image:467d.gif|left|200px]] | + | [[Image:467d.gif|left|200px]] |
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| - | '''The structure of a decamer forming a four-way junction''' | + | {{Structure |
| + | |PDB= 467d |SIZE=350|CAPTION= <scene name='initialview01'>467d</scene>, resolution 2.16Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''The structure of a decamer forming a four-way junction''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 467D is a [ | + | 467D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=467D OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:[http:// | + | Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504723 10504723] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Coll, M.]] | [[Category: Coll, M.]] | ||
[[Category: Ortiz-Lombardia, M.]] | [[Category: Ortiz-Lombardia, M.]] | ||
[[Category: four-way-junction]] | [[Category: four-way-junction]] | ||
| - | [[Category: g:a | + | [[Category: g:a mismatch]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:34 2008'' |
Revision as of 17:08, 20 March 2008
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| , resolution 2.16Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of a decamer forming a four-way junction
Overview
DNA recombination is a universal biological event responsible both for the generation of genetic diversity and for the maintenance of genome integrity. A four-way DNA junction, also termed Holliday junction, is the key intermediate in nearly all recombination processes. This junction is the substrate of recombination enzymes that promote branch migration or catalyze its resolution. We have determined the crystal structure of a four-way DNA junction by multiwavelength anomalous diffraction, and refined it to 2.16 A resolution. The structure has two-fold symmetry, with pairwise stacking of the double-helical arms, which form two continuous B-DNA helices that run antiparallel, cross in a right-handed way, and contain two G-A mismatches. The exchanging backbones form a compact structure with strong van der Waals contacts and hydrogen bonds, implying that a conformational change must occur for the junction to branch-migrate or isomerize. At the branch point, two phosphate groups from one helix occupy the major groove of the other one, establishing sequence-specific hydrogen bonds. These interactions, together with different stacking energies and steric hindrances, explain the preference for a particular junction stacked conformer.
About this Structure
467D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DNA Holliday junction., Ortiz-Lombardia M, Gonzalez A, Eritja R, Aymami J, Azorin F, Coll M, Nat Struct Biol. 1999 Oct;6(10):913-7. PMID:10504723
Page seeded by OCA on Thu Mar 20 19:08:34 2008
