485d
From Proteopedia
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| - | [[Image:485d.gif|left|200px]] | + | [[Image:485d.gif|left|200px]] |
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| - | '''A SULFATE POCKET FORMED BY THREE GOU PAIRS IN THE STRUCTURE OF A NONAMERIC RNA''' | + | {{Structure |
| + | |PDB= 485d |SIZE=350|CAPTION= <scene name='initialview01'>485d</scene>, resolution 0.97Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A SULFATE POCKET FORMED BY THREE GOU PAIRS IN THE STRUCTURE OF A NONAMERIC RNA''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 485D is a [ | + | 485D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=485D OCA]. |
==Reference== | ==Reference== | ||
| - | A sulfate pocket formed by three GoU pairs in the 0.97 A resolution X-ray structure of a nonameric RNA., Masquida B, Sauter C, Westhof E, RNA. 1999 Oct;5(10):1384-95. PMID:[http:// | + | A sulfate pocket formed by three GoU pairs in the 0.97 A resolution X-ray structure of a nonameric RNA., Masquida B, Sauter C, Westhof E, RNA. 1999 Oct;5(10):1384-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10573129 10573129] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Masquida, B.]] | [[Category: Masquida, B.]] | ||
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[[Category: sulfate ion]] | [[Category: sulfate ion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:45 2008'' |
Revision as of 17:08, 20 March 2008
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| , resolution 0.97Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
A SULFATE POCKET FORMED BY THREE GOU PAIRS IN THE STRUCTURE OF A NONAMERIC RNA
Overview
The crystal structure of the RNA duplex [r(CGUGAUCG)dC]2 has been solved at a resolution of 0.97 A. The model has been refined to R-work and R-free of 14.88% and 19.54% for 23,838 independent reflections. The base-pairing scheme forces the 5'-rC to be excluded from the helix and to be disordered. In the crystals, the sequence promotes the formation of two GoU wobble pairs that cluster around a crystallographic threefold axis in two different ways. In the first contact type, the GoU pairs are exclusively surrounded by water molecules, whereas in the other contact type, the three amino groups of the guanine residues of the symmetry-related GoU pairs trap a sulfate ion. This work provides the first example of the interaction of a GoU pair with a sulfate ion in a helical context. Despite the negative charge on the polynucleotide backbone, the guanine amino N2 is able to attract negatively charged groups that could, in the folding of complex RNA molecules, belong to a negative phosphodiester group from a neighboring strand and, in a RNA-protein complex, to a negative carboxyl group of an aspartate or glutamate side chain.
About this Structure
485D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A sulfate pocket formed by three GoU pairs in the 0.97 A resolution X-ray structure of a nonameric RNA., Masquida B, Sauter C, Westhof E, RNA. 1999 Oct;5(10):1384-95. PMID:10573129
Page seeded by OCA on Thu Mar 20 19:08:45 2008
Categories: Protein complex | Masquida, B. | Sauter, C. | Westhof, E. | SO4 | Dna/rna hybrid | Rna | Sulfate ion
