4cln
From Proteopedia
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| - | [[Image:4cln.gif|left|200px]] | + | [[Image:4cln.gif|left|200px]] |
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| - | '''STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTER REFINED AT 2.2-ANGSTROMS RESOLUTION''' | + | {{Structure |
| + | |PDB= 4cln |SIZE=350|CAPTION= <scene name='initialview01'>4cln</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTER REFINED AT 2.2-ANGSTROMS RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4CLN is a [ | + | 4CLN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CLN OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2-A resolution., Taylor DA, Sack JS, Maune JF, Beckingham K, Quiocho FA, J Biol Chem. 1991 Nov 15;266(32):21375-80. PMID:[http:// | + | Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2-A resolution., Taylor DA, Sack JS, Maune JF, Beckingham K, Quiocho FA, J Biol Chem. 1991 Nov 15;266(32):21375-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1939171 1939171] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: calcium binding protein]] | [[Category: calcium binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:09:13 2008'' |
Revision as of 17:09, 20 March 2008
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| , resolution 2.2Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF A RECOMBINANT CALMODULIN FROM DROSOPHILA MELANOGASTER REFINED AT 2.2-ANGSTROMS RESOLUTION
Overview
The crystal structure of calmodulin (Mr 16,700, 148 residues) from Drosophila melanogaster as expressed in a bacterial system has been determined and refined at 2.2-A resolution. Starting with the structure of mammalian calmodulin, we produced an extensively refitted and refined model with a conventional crystallographic R value of 0.197 for the 5,239 reflections (F greater than or equal to 2 sigma (F)) within the 10.0-2.2-A resolution range. The model includes 1,164 protein atoms, 4 calcium ions, and 78 water molecules and has root mean square deviations from standard values of 0.018 A for bond lengths and 0.043 A for angle distances. The overall structure is similar to mammalian calmodulin, with a seven-turn central helix connecting the two calcium-binding domains. The "dumb-bell" shaped molecule contains seven alpha-helices and four "EF hand" calcium-binding sites. Although the amino acid sequences of mammalian and Drosophila calmodulins differ by only three conservative amino acid changes, the refined model reveals a number of significant differences between the two structures. Superimposition of the structures yields a root mean square deviation of 1.22 A for the 1,120 equivalent atoms. The calcium-binding domains have a root mean square deviation of 0.85 A for the 353 equivalent atoms. There are also differences in the amino terminus, the bend of the central alpha-helix, and the orientations of some of the side chains.
About this Structure
4CLN is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2-A resolution., Taylor DA, Sack JS, Maune JF, Beckingham K, Quiocho FA, J Biol Chem. 1991 Nov 15;266(32):21375-80. PMID:1939171
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