This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4cro
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:4cro.gif|left|200px]] | + | [[Image:4cro.gif|left|200px]] |
| - | + | ||
| - | '''PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX''' | + | {{Structure |
| + | |PDB= 4cro |SIZE=350|CAPTION= <scene name='initialview01'>4cro</scene>, resolution 3.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 4CRO is a [ | + | 4CRO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CRO OCA]. |
==Reference== | ==Reference== | ||
| - | Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:[http:// | + | Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2146682 2146682] |
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:09:17 2008'' |
Revision as of 17:09, 20 March 2008
| |||||||
| , resolution 3.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX
Overview
The structure of a complex of bacteriophage lambda Cro protein with a 17-base-pair operator has been determined at 3.9-A resolution. Isomorphous derivatives obtained by the synthesis of site-specific iodinated DNA oligomers were of critical importance in solving the structure. The crystal structure contains three independent Cro-operator complexes that have very similar, although not necessarily identical, conformations. In the complex, the protein dimer undergoes a large conformational change relative to the crystal structure of the free protein. One monomer rotates by about 40 degrees relative to the other, this being accomplished primarily by a twisting of the two beta-sheet strands that connect one monomer with the other. In the complex, the DNA is bent by about 40 degrees into the shape of a boomerang but maintains essentially Watson-Crick B-form. In contrast to other known protein-DNA complexes, the DNA is not stacked end-to-end. The structure confirms the general features of the model previously proposed for the interaction of Cro with DNA.
About this Structure
4CRO is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:2146682
Page seeded by OCA on Thu Mar 20 19:09:17 2008
