4xgy
From Proteopedia
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacterium Vibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space group P43212, with unit-cell parameters a = b = 63.35, c = 125.90 A, were obtained by vapour diffusion in hanging drops and the structure was refined to an Rfree of 16.7% at 1.5 A resolution. The structure shows a CDR loop of the antibody on the GFP scaffold. | ||
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| + | The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.,Chen Y, Huang X, Wang R, Wang S, Shi N Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):913-8. doi:, 10.1107/S2053230X15008845. Epub 2015 Jun 27. PMID:26144238<ref>PMID:26144238</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:10, 22 July 2015
GFP based antibody (fluorobody)
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Categories: Chen, Y G | Shi, N | Wang, S H | Cdr3 | Fluorescent protein | Fluorobody | Gfp
