5mht
From Proteopedia
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| - | [[Image:5mht.gif|left|200px]] | + | [[Image:5mht.gif|left|200px]] |
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| - | '''TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY''' | + | {{Structure |
| + | |PDB= 5mht |SIZE=350|CAPTION= <scene name='initialview01'>5mht</scene>, resolution 2.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nicotinate_N-methyltransferase Nicotinate N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.7 2.1.1.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 5MHT is a [ | + | 5MHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHT OCA]. |
==Reference== | ==Reference== | ||
| - | A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase., O'Gara M, Roberts RJ, Cheng X, J Mol Biol. 1996 Nov 8;263(4):597-606. PMID:[http:// | + | A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase., O'Gara M, Roberts RJ, Cheng X, J Mol Biol. 1996 Nov 8;263(4):597-606. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8918941 8918941] |
[[Category: Haemophilus haemolyticus]] | [[Category: Haemophilus haemolyticus]] | ||
[[Category: Nicotinate N-methyltransferase]] | [[Category: Nicotinate N-methyltransferase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:12:29 2008'' |
Revision as of 17:12, 20 March 2008
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| , resolution 2.700Å | |||||||
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| Ligands: | |||||||
| Activity: | Nicotinate N-methyltransferase, with EC number 2.1.1.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY
Overview
The crystal structure of HhaI methyltransferase complexed with non-palindromic duplex DNA, containing a hemimethylated recognition sequence, and with the cofactor analog S-adenosyl-L-homocysteine (AdoHcy), has been determined. The structure provides an explanation for the stronger affinities of DNA methyltransferases for hemimethylated DNA than for unmethylated or fully methylated DNA in the presence of AdoHcy. The unmethylated target 2'-deoxycytidine flips out of the DNA helix and the CH group at position 5 makes van der Waals' contacts with the sulfur atom of AdoHcy. Selectivity/preference for hemimethylated over fully methylated DNA may thus reflect interactions among the chemical substituent (H or CH3) at the C5 position of the flipped cytosine, protein and the bound AdoHcy. The 5-methyl-2'-deoxycytidine on the complementary strand remains in the DNA helix, with the methyl group almost perpendicular to the carboxylate group of Glu239, which is part of the sequence recognition loop. Thus, selectivity/preference for hemimethylated over unmethylated DNA appears to result largely from van der Waals' contacts between the planar Glu239 carboxylate and the methyl group of the 5-methyl-2'-deoxycytidine. Furthermore, the positive electrostatic potential originating from the bound AdoHcy extends to the DNA phosphate groups flanking the flipped cytosine. The increased binding to DNA by long-range electrostatic interactions should also occur with the methyl donor S-adenosyl-L-methionine.
About this Structure
5MHT is a Single protein structure of sequence from Haemophilus haemolyticus. Full crystallographic information is available from OCA.
Reference
A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase., O'Gara M, Roberts RJ, Cheng X, J Mol Biol. 1996 Nov 8;263(4):597-606. PMID:8918941
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